Clinical validation of the lyra direct HSV 1+2/VZV assay for simultaneous detection and differentiation of three herpesviruses in cutaneous and mucocutaneous lesions Journal Article

   

Authors:	Fan, F.; Stiles, J.; Mikhlina, A.; Lu, X.; Esther Babady, N.; Tang, Y. W.
Article Title:	Clinical validation of the lyra direct HSV 1+2/VZV assay for simultaneous detection and differentiation of three herpesviruses in cutaneous and mucocutaneous lesions
Abstract:	We evaluated the Lyra Direct HSV 1+2/VZV multiplex real-time PCR assay for the detection and differentiation of herpes simplex virus 1 (HSV-1), HSV-2, and varicella-zoster virus (VZV) on 695 consecutive cutaneous and mucocutaneous lesion specimens. The intra-assay and interassay coefficient of variation values for the Lyra assay were 0.29 to 1.30% and 2.33 to 2.61%, respectively. The sensitivities, specificities, and positive and negative predictive values were 93.4 to 95.0%, 96.1 to 96.8%, 78.0 to 80.3%, and 99.0 to 99.1%, respectively, in comparison to those of viral culture. The values were further improved when a resolution analysis was performed with a laboratory-developed PCR assay. Copyright
Journal Title:	Journal of Clinical Microbiology
Volume:	52
Issue:	10
ISSN:	0095-1137
Publisher:	American Society for Microbiology
Date Published:	2014-10-01
Start Page:	3799
End Page:	3801
Language:	English
DOI:	10.1128/jcm.02098-14
PROVIDER:	scopus
PUBMED:	25078915
PMCID:	PMC4187752
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84907185205&partnerID=40&md5=de6fc564e4f87c1b4c4cd98c758da310
Notes:	Export Date: 1 October 2014 -- Source: Scopus

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