Evaluation of BioFire Respiratory Panel 2 plus for detection of Bordetella pertussis in nasopharyngeal swab specimens from children with clinically suspected pertussis Journal Article
| Authors: | Li, C.; Huang, C.; Zhang, R.; Wang, H.; Tian, S.; Tang, Y. W.; Deng, J. |
| Article Title: | Evaluation of BioFire Respiratory Panel 2 plus for detection of Bordetella pertussis in nasopharyngeal swab specimens from children with clinically suspected pertussis |
| Abstract: | The objective of this study was to compare the performances of BioFire Respiratory Panel 2 (RP2) plus, quantitative real-time PCR (qPCR), and culture for the detection of Bordetella pertussis in nasopharyngeal swab (NPS) specimens. Consecutive NPS specimens were collected from patients with clinically suspected pertussis from 1 March 1 to 31 July 2018 in Shenzhen Children’s Hospital. All the specimens were tested in parallel by RP2 plus, qPCR, and culture methods. A total of 464 children were enrolled in this study. The positive pertussis rates of culture, RP2 plus, and qPCR were 23.1%, 39.0%, and 38.4%, respectively. Compared to the combined reference standard, the sensitivity, specificity, positive predictive value, and negative predictive values were, respectively, 56.6% (95% confidence interval [CI], 49.2 to 63.7%), 100% (98.3 to 100%), 100% (95.7 to 100%), and 77.0% (72.2 to 81.2%) for culture, 89.9% (84.5 to 93.7%), 96.0% (92.8 to 97.9%), 93.9% (89.1 to 96.8%), and 93.3% (89.5 to 95.8%) for RP2 plus, and 86.8% (80.9 to 91.1%), 94.9% (91.4 to 97.1%), 92.1% (86.9 to 95.5%), and 91.3% (87.2 to 94.2%) for qPCR. The most prevalent codetected pathogen was human rhinovirus/enterovirus (n = 99, 52.4%), followed by parainfluenza virus (n =32, 16.9%) and respiratory syncytial virus (n = 29, 15.3%), in children with B. pertussis present, which was consistent with the top three pathogens previously found in children with B. pertussis absent. Turnaround times for RP2 plus, qPCR, and culture were 2 h, 8 h, and 120 h, respectively. RP2 plus quickly and accurately detected B. pertussis, providing valuable information for an early clinical diagnosis and optimal choice of therapy. Copyright © 2023 Li et al. |
| Keywords: | child; genetics; children; bordetella pertussis; pertussis; nasopharynx; whooping cough; procedures; multiplex polymerase chain reaction; humans; human; human respiratory syncytial virus; respiratory pathogens; biofire respiratory panel 2 plus; respiratory syncytial virus, human |
| Journal Title: | Microbiology Spectrum |
| Volume: | 11 |
| Issue: | 1 |
| ISSN: | 2165-0497 |
| Publisher: | American Society for Microbiology |
| Date Published: | 2023-01-01 |
| Start Page: | e01806-22 |
| Language: | English |
| DOI: | 10.1128/spectrum.01806-22 |
| PUBMED: | 36602355 |
| PROVIDER: | scopus |
| PMCID: | PMC9927272 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 March 2023 -- Source: Scopus |
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