Isolation of human t lymphocytes by a negative selection procedure Journal Article
| Authors: | Chiao, J. W.; Good, R. A. |
| Article Title: | Isolation of human t lymphocytes by a negative selection procedure |
| Abstract: | A single step method to isolate human T lymphocytes was achieved using a rosette technique to deplete non-T lymphocytes. Erythrocytes coated simultaneously with IgG and complement were used to form rosettes with cells bearing such receptors during a short incubation period and the resetting cells removed by gradient centrifugation. T lymphocytes were isolated (>96% T cells) without exposing to much experimental manipulations and in good recovery (mean 60%, range 40-75%). © 1979 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. |
| Journal Title: | Immunological Investigations |
| Volume: | 8 |
| Issue: | 1 |
| ISSN: | 0882-0139 |
| Publisher: | Informa Healthcare |
| Date Published: | 1979-01-01 |
| Start Page: | 49 |
| End Page: | 54 |
| Language: | English |
| DOI: | 10.3109/08820137909044705 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 1 October 2014 -- Source: Scopus |
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