Isolation of human t lymphocytes by a negative selection procedure Journal Article


Authors: Chiao, J. W.; Good, R. A.
Article Title: Isolation of human t lymphocytes by a negative selection procedure
Abstract: A single step method to isolate human T lymphocytes was achieved using a rosette technique to deplete non-T lymphocytes. Erythrocytes coated simultaneously with IgG and complement were used to form rosettes with cells bearing such receptors during a short incubation period and the resetting cells removed by gradient centrifugation. T lymphocytes were isolated (>96% T cells) without exposing to much experimental manipulations and in good recovery (mean 60%, range 40-75%). © 1979 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Journal Title: Immunological Investigations
Volume: 8
Issue: 1
ISSN: 0882-0139
Publisher: Informa Healthcare  
Date Published: 1979-01-01
Start Page: 49
End Page: 54
Language: English
DOI: 10.3109/08820137909044705
PROVIDER: scopus
DOI/URL:
Notes: Export Date: 1 October 2014 -- Source: Scopus
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