Probing motions between equivalent RNA domains using magnetic field induced residual dipolar couplings: Accounting for correlations between motions and alignment Journal Article

  

Authors:	Zhang, Q.; Throolin, R.; Pitt, S. W.; Serganov, A.; Al-Hashimi, H. M.
Article Title:	Probing motions between equivalent RNA domains using magnetic field induced residual dipolar couplings: Accounting for correlations between motions and alignment
Abstract:	Approaches developed thus for extracting structural and dynamical information from RDCs have rested on the assumption that motions do not affect molecular alignment. However, it is well established that molecular alignment in ordered media is dependent on conformation, and slowly interconverting conformational substates may exhibit different alignment properties. Neglecting these correlation effects can lead to aberrations in the structural and dynamical analysis of RDCs and diminish the utility of RDCs in probing motions between domains having similar alignment propensities. Here, we introduce a new approach based on measurement of magnetic field induced residual dipolar couplings in nucleic acids which can explicitly take into account such correlations and demonstrate measurements of motions between two "magnetically equivalent" domains in the transactivation response element (TAR) RNA. Copyright © 2003 American Chemical Society.
Keywords:	molecular dynamics; rna; measurement; transactivation; response elements; nucleic acid conformation; motion; dipole; calculation; magnetics; nucleic acid; nuclear magnetic resonance, biomolecular; magnetic field; trans-activation (genetics); article
Journal Title:	Journal of the American Chemical Society
Volume:	125
Issue:	35
ISSN:	0002-7863
Publisher:	American Chemical Society
Date Published:	2003-09-03
Start Page:	10530
End Page:	10531
Language:	English
DOI:	10.1021/ja0363056
PUBMED:	12940730
PROVIDER:	scopus
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-0042355704&partnerID=40&md5=23f43c94fe520520e521d2b5c614ce18
Notes:	Export Date: 12 September 2014 -- Source: Scopus

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