Translocation of β-catenin into the nucleus independent of interactions with FG-rich nucleoporins Journal Article
| Authors: | Suh, E. K.; Gumbiner, B. M. |
| Article Title: | Translocation of β-catenin into the nucleus independent of interactions with FG-rich nucleoporins |
| Abstract: | β-Catenin nuclear import has been found to be independent of classical nuclear localization signal (NLS) nuclear import factors. Here, we test the hypothesis that β-catenin interacts directly with nuclear pore proteins to mediate its own transport. We show that β-catenin, unlike importin-β, does not interact detectably with Phe/Gly(FG)-repeat-rich nuclear pore proteins or nucleoporins (Nups). Moreover, unlike NLS-containing proteins, β-catenin nuclear import is not inhibited by wheat germ agglutinin (WGA) or excess importin-β. These results suggest β-catenin nuclear translocation does not involve direct interactions with FG-Nups. However, β-catenin has two regions that can target it to the nucleus, and its import is cold sensitive, indicating that β-catenin nuclear import is still an active process. Transport is blocked by a soluble form of the C-cadherin cytoplasmic domain, suggesting that masking of the nuclear targeting signal may be a mechanism of regulating β-catenin subcellular localization. © 2003 Elsevier Inc. All rights reserved. |
| Keywords: | controlled study; nonhuman; animal cell; animals; protein interaction; cercopithecus aethiops; cos cells; nuclear proteins; recombinant proteins; protein transport; cytoplasm; active transport, cell nucleus; chromosome translocation; temperature; protein structure, tertiary; trans-activators; cell nucleus; beta catenin; biological transport; cytosol; nucleotide repeat; nuclear localization signals; cytoskeletal proteins; xenopus laevis; nuclear pore complex proteins; hypothesis; dipeptides; beta-galactosidase; wheat germ; β-catenin; xenopus proteins; nuclear pore; nucleoporin; nuclear pore complex; karyopherin beta; beta karyopherins; nuclear import; concanavalin a; wheat germ agglutinin; triticum aestivum; priority journal; article; importin-β; nls; wheat germ agglutinins |
| Journal Title: | Experimental Cell Research |
| Volume: | 290 |
| Issue: | 2 |
| ISSN: | 0014-4827 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2003-11-01 |
| Start Page: | 447 |
| End Page: | 456 |
| Language: | English |
| DOI: | 10.1016/s0014-4827(03)00370-7 |
| PUBMED: | 14568002 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Source: Scopus |
