β-catenin directly induces expression of the Siamois gene, and can initiate signaling indirectly via a membrane-tethered form Conference Paper
| Authors: | Nelson, R. W.; Gumbiner, B. M. |
| Title: | β-catenin directly induces expression of the Siamois gene, and can initiate signaling indirectly via a membrane-tethered form |
| Conference Title: | Morphogenesis: Cellular Interactions |
| Abstract: | β-catenin is shown to directly induce the expression of siamois (a homeobox-containing gene involved in axial patterning) in a cell-autonomous, protein synthesis independent manner. Siamois can thus be considered a direct target of β-catenin signaling in Xenopus. Expression of a portion of the armadillo repeat region of β-catenin via a membrane-tethered fusion protein is shown to give similar levels of siamois induction and axis duplication as a free, untethered form. Reduction of endogenous free β-catenin levels by overexpression of C-cadherin leads to complete inhibition of signaling by the membrane-tethered repeat region. Since the membrane-tethered repeat region is unlikely to be bound up by C-cadherin, these results show that the membrane-tethered β-catenin relies on endogenous β-catenin for signaling. We propose that the membrane-tethered construct acts by titrating a cytoplasmic inhibitor of β-catenin signaling. |
| Keywords: | signal transduction; controlled study; protein expression; nonhuman; conference paper; animals; gene expression; morphogenesis; homeodomain proteins; embryo, nonmammalian; gene expression regulation, developmental; hybrid protein; reverse transcriptase polymerase chain reaction; recombinant proteins; cytoplasm; base sequence; trans-activators; gene induction; beta catenin; cadherin; cadherins; repetitive sequences, nucleic acid; armadillo; cytoskeletal proteins; xenopus; xenopus proteins; homeobox; blastomeres |
| Journal Title | Annals of the New York Academy of Sciences |
| Volume: | 857 |
| Conference Dates: | 1997 Oct 20-23 |
| Conference Location: | Bethesda, MD |
| ISBN: | 0077-8923 |
| Publisher: | John Wiley & Sons |
| Date Published: | 1998-10-01 |
| Start Page: | 86 |
| End Page: | 98 |
| Language: | English |
| PUBMED: | 9917834 |
| PROVIDER: | scopus |
| DOI: | 10.1111/j.1749-6632.1998.tb10109.x |
| DOI/URL: | |
| Notes: | Article in "Morphogenesis: Cellular Interactions" (ISBN: 9781573311311) -- Conference Paper -- Export Date: 12 December 2016 -- Source: Scopus |
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