Crystal structure of a human Mob1 protein: Toward understanding Mob-regulated cell cycle pathways Journal Article
| Authors: | Stavridi, E. S.; Harris, K. G.; Huyen, Y.; Bothos, J.; Verwoerd, P. M.; Stayrook, S. E.; Pavletich, N. P.; Jeffrey, P. D.; Luca, F. C. |
| Article Title: | Crystal structure of a human Mob1 protein: Toward understanding Mob-regulated cell cycle pathways |
| Abstract: | The Mob protein family comprises a group of highly conserved eukaryotic proteins whose founding member functions in the mitotic exit network. At the molecular level, Mob proteins act as kinase-activating subunits. We cloned a human Mob1 family member, Mob1A, and determined its three-dimensional structure by X-ray crystallography. The core of Mob1A consists of a four-helix bundle that is stabilized by a bound zinc atom. The N-terminal helix of the bundle is solvent exposed and together with adjacent secondary structure elements forms an evolutionarily conserved surface with a strong negative electrostatic potential. Several conditional mutant alleles of S. cerevisiae MOB1 target this surface and decrease its net negative charge. Interestingly, the kinases with which yeast Mob proteins interact have two conserved basic regions within their N-terminal lobe. Thus, Mob proteins may regulate their target kinases through electrostatic interactions mediated by conserved charged surfaces. |
| Keywords: | signal transduction; controlled study; unclassified drug; nonhuman; protein conformation; protein function; cell cycle protein; cell cycle proteins; allele; cell cycle; nerve tissue proteins; protein stability; membrane proteins; molecular cloning; regulatory mechanism; amino acid sequence; molecular sequence data; sequence homology, amino acid; amino terminal sequence; saccharomyces cerevisiae; eukaryota; nucleotide sequence; phosphoproteins; crystal structure; crystallography, x-ray; protein structure, tertiary; molecular interaction; saccharomyces cerevisiae proteins; protein secondary structure; protein structure; zinc; protein family; x ray crystallography; protein interaction mapping; phosphotransferase; protein tertiary structure; fungal protein; electricity; mutant; solvent; saccharomyces; humans; priority journal; article; protein mob1 |
| Journal Title: | Structure |
| Volume: | 11 |
| Issue: | 9 |
| ISSN: | 0969-2126 |
| Publisher: | Cell Press |
| Date Published: | 2003-09-01 |
| Start Page: | 1163 |
| End Page: | 1170 |
| Language: | English |
| DOI: | 10.1016/s0969-2126(03)00182-5 |
| PUBMED: | 12962634 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Molecular Sequence Numbers: GENBANK: AK023321, BC038112; -- Source: Scopus |
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