Vaccinia topoisomerase mutants illuminate roles for Phe59, Gly73, Gln69 and Phe215 Journal Article
| Authors: | Tian, L.; Shuman, S. |
| Article Title: | Vaccinia topoisomerase mutants illuminate roles for Phe59, Gly73, Gln69 and Phe215 |
| Abstract: | Vaccinia topoisomerase provides a model system for structure-function analysis of the type IB topoisomerase family. Here we performed an alanine scan of eight positions in the β4 and β5 strands of the N-terminal domain (Leu57, Ile58, Phe59, Val60, Gly61, Ser62, Gln69 and Gly73) and eight positions in the α8-α9 loop of the C-terminal catalytic domain (Ser241, Ile242, Ser243, Pro244, Leu245, Pro246, Ser247, and Pro248). Mutants F59A, G73A, and Q69A displayed rate defects in relaxing supercoiled DNA that were attributed to effects on DNA binding rather than transesterification chemistry. Replacing Gln69 conservatively with Asn, Glu or Lys failed to restore relaxation activity. Gln69 is located along a concave DNA-binding surface of the N-terminal domain and it makes direct contact with the + 2A base of the 5′-CCCTT/3-GGGAA target site for DNA cleavage. Gly73 is located at the junction between the N-terminal domain and catalytic domain and it is likely to act as a swivel for the large domain movements that coordinate DNA ingress and closure of the topoisomerase clamp around the duplex. Previous alanine scanning had identified Phe215 in helix α7 of the catalytic domain as contributing to DNA relaxation activity. Here we find that F215L resembles F215A in its diminished relaxation activity and its sensitivity to inhibition by salt. The Phe215 side chain makes van der Waals contacts to Ile98, Met121 and Phe101, which we propose stabilize a three helix bundle and promote clamp closure. © 2006 Elsevier Inc. All rights reserved. |
| Keywords: | controlled study; mutation; nonhuman; protein conformation; enzyme inhibition; amino acid substitution; serine; carboxy terminal sequence; protein dna binding; structure activity relation; dna; amino acid sequence; molecular sequence data; amino terminal sequence; vaccinia virus; binding site; dna, viral; models, molecular; isoleucine; molecular interaction; alanine; catalysis; nucleic acid conformation; leucine; catalytic domain; sodium chloride; mutagenesis; glutamine; lysine; glycine; asparagine; transesterification; phenylalanine; valine; dna cleavage; virus mutant; dna topoisomerase; proline; magnesium; poxvirus; poxviridae; dna topoisomerases; sequence specificity; topoisomerase ib |
| Journal Title: | Virology |
| Volume: | 359 |
| Issue: | 2 |
| ISSN: | 0042-6822 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2007-03-01 |
| Start Page: | 466 |
| End Page: | 476 |
| Language: | English |
| DOI: | 10.1016/j.virol.2006.08.056 |
| PUBMED: | 17059840 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 2" - "Export Date: 17 November 2011" - "CODEN: VIRLA" - "Source: Scopus" |
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