A common site on TBP for transcription by RNA polymerases II and III Journal Article
| Authors: | Schröder, O.; Bryant, G. O.; Geiduschek, E. P.; Berk, A. J.; Kassavetis, G. A. |
| Article Title: | A common site on TBP for transcription by RNA polymerases II and III |
| Abstract: | The TATA-binding protein (TBP) is involved in all nuclear transcription. We show that a common site on TBP is used for transcription initiation complex formation by RNA polymerases (pols) II and III. TBP, the transcription factor IIB (TFIIB)-related factor Brf1 and the pol III-specific factor Bdp1 constitute TFIIIB. A photochemical cross-linking approach was used to survey a collection of human TBP surface residue mutants for their ability to form TFIIIB-DNA complexes reliant on only the TFIIB-related part of Brfl. Mutations impairing complex formation and transcription were identified and mapped on the surface of TBP. The most severe effects were observed for mutations in the C-terminal stirrup of TBP, which is the principal site of interaction between TBP and TFIIB. Structural modeling of the Brf1-TBP complex and comparison with its TFIIB-TBP analog further rationalizes the close resemblance of the TBP interaction with the N-proximal part of Brf1 and TFIIB, and establishes the conserved usage of a TBP surface in pol II and pol III transcription for a conserved function in the initiation of transcription. |
| Keywords: | controlled study; unclassified drug; mutation; protein function; complex formation; carboxy terminal sequence; protein protein interaction; transcription factor; transcription, genetic; amino acid sequence; molecular sequence data; amino terminal sequence; saccharomyces cerevisiae; binding sites; saccharomyces cerevisiae proteins; rna polymerase ii; rna transcription; transcription initiation site; photochemistry; cross linking; tata-binding protein associated factors; dna directed rna polymerase iii; rna polymerase iii; tata binding protein; tata-box binding protein; human; priority journal; article; tbp; brf1; rna polymerases iii and ii; tfiib; transcriptional initiation; transcription factor 2b related factor brf1; transcription factor iib; transcription factor tfiib; transcription factor tfiiib |
| Journal Title: | EMBO Journal |
| Volume: | 22 |
| Issue: | 19 |
| ISSN: | 0261-4189 |
| Publisher: | Wiley Blackwell |
| Date Published: | 2003-10-01 |
| Start Page: | 5115 |
| End Page: | 5124 |
| Language: | English |
| DOI: | 10.1093/emboj/cdg476 |
| PUBMED: | 14517249 |
| PROVIDER: | scopus |
| PMCID: | PMC204460 |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Source: Scopus |
