Crystal structure of Rcl1, an essential component of the eukaryal pre-rRNA processosome implicated in 18s rRNA biogenesis Journal Article

   

Authors:	Tanaka, N.; Smith, P.; Shuman, S.
Article Title:	Crystal structure of Rcl1, an essential component of the eukaryal pre-rRNA processosome implicated in 18s rRNA biogenesis
Abstract:	Rcl1 is an essential nucleolar protein required for U3 snoRNA-guided pre-rRNA processing at sites flanking the 18S rRNA sequence. A potential catalytic role for Rcl1 during pre-rRNA cleavage has been suggested based on its primary structure similarity to RNA 3′-terminal phosphate cyclase (Rtc) enzymes, which perform nucleotidyl transfer and phosphoryl transfer reactions at RNA ends. Here, we report the 2.6 Å crystal structure of a biologically active yeast Rcl1, which illuminates its modular 4-domain architecture and overall homology with RNA cyclases while revealing numerous local differences that account for why Rtcs possess metal-dependent adenylyltransferase activity and Rcls do not. A conserved oxyanion-binding site in Rcl1 was highlighted for possible catalytic or RNA-binding functions. However, the benign effects of mutations in and around the anion site on Rcl1 activity in vivo militate against such a role. Copyright © 2011 RNA Society.
Keywords:	controlled study; unclassified drug; missense mutation; nonhuman; binding affinity; protein domain; protein localization; animal cell; nuclear protein; animal experiment; in vivo study; enzyme activity; nuclear proteins; rna binding protein; rna; amino acid sequence; molecular sequence data; saccharomyces cerevisiae; binding site; crystal structure; crystallography, x-ray; protein structure, tertiary; yeast; saccharomyces cerevisiae proteins; catalysis; ribosome rna; rna processing; protein structure, secondary; mutagenesis; metal; eukaryote; kluyveromyces; kluyveromyces lactis; rna binding; rna precursors; biogenesis; adenylyltransferase; rna 18s; illumination; rna cyclase; rrna processing; anion; enzyme rtc; oxyanion; protein rcl1; ribosome rna precursor; rna precursor; transferase; rna, ribosomal, 18s
Journal Title:	RNA
Volume:	17
Issue:	4
ISSN:	1355-8382
Publisher:	Cold Spring Harbor Laboratory Press
Date Published:	2011-04-01
Start Page:	595
End Page:	602
Language:	English
DOI:	10.1261/rna.2571811
PUBMED:	21367972
PROVIDER:	scopus
PMCID:	PMC3062171
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-79952999900&partnerID=40&md5=4824cdd4106a20a5dcd5d3c9165487d9
Notes:	--- - "Export Date: 23 June 2011" - "CODEN: RNARF" - "Source: Scopus"

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