Identification and characterization of two new human mu opioid receptor splice variants, hMOR-1O and hMOR-1X Journal Article
| Authors: | Pan, Y. X.; Xu, J.; Mahurter, L.; Xu, M.; Gilbert, A. K.; Pasternak, G. W. |
| Article Title: | Identification and characterization of two new human mu opioid receptor splice variants, hMOR-1O and hMOR-1X |
| Abstract: | The mouse gene encoding the mu opioid receptor, Oprm, undergoes extensive alternatively splicing, with 14 variants having been identified. However, only one variant of human mu opioid receptor gene (Oprm), MOR-1A, has been described. We now report two novel splice variants of the human Oprm gene, hMOR-1O and hMOR-1X. The full-length cDNAs of hMOR-1O and hMO-1X contained the same exons 1, 2, and 3 as the original hMOR-1, but with exon O or exon X as the alternative fourth exon, respectively. Northern blots revealed several bands with the exon O probe in both human neuroblastoma BE(2)C cells and human brain and a single band (5.5kb) with the exon X probe in selected human brain regions. When transfected into CHO cells, both variants showed high selectivity for mu opioids in binding assays. These two new human mu opioid receptors are the first human MOR-1 variants containing new exons and suggest that the complex splicing present in mice may extend to humans. © 2003 Elsevier Science (USA). All rights reserved. |
| Keywords: | human cell; exon; exons; animals; mice; tumor cells, cultured; transfection; amino acid sequence; molecular sequence data; sequence homology, amino acid; genetic transfection; brain; neuroblastoma cell; gene identification; nucleotide sequence; recombinant proteins; alternative splicing; alternative rna splicing; base sequence; dna sequence; protein variant; mu opiate receptor; brain region; molecular probe; receptors, opioid, mu; receptor binding; complementary dna; dna, complementary; northern blotting; opioid receptor; splicing; cho cell; cho cells; cricetinae; variant; binding assay; mu opioid receptor; variation (genetics); humans; human; priority journal; article |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 301 |
| Issue: | 4 |
| ISSN: | 0006-291X |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2003-02-21 |
| Start Page: | 1057 |
| End Page: | 1061 |
| Language: | English |
| DOI: | 10.1016/s0006-291x(03)00089-5 |
| PUBMED: | 12589820 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Molecular Sequence Numbers: GENBANK: AY036622, AY036623; -- Source: Scopus |
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