Characterization of vascular adhesion molecules that may facilitate progenitor homing in the post-natal mouse thymus Journal Article
| Authors: | Lepique, A. P.; Palencia, S.; Irjala, H.; Petrie, H. T. |
| Article Title: | Characterization of vascular adhesion molecules that may facilitate progenitor homing in the post-natal mouse thymus |
| Abstract: | T cell progenitors derive from the bone marrow but must migrate via bloodstream to the thymus in order to differentiate. The mechanism by which the thymus recruits progenitors from the blood is unknown. It is known, however, that there are receptive and refractory periods for progenitor recruitment and that when cells are imported, they enter the thymus through post-capillary venules. Therefore, recruitment is an active process temporally and spatially regulated. In order to characterize the mechanism of recruitment, we evaluated vascular signals known to regulate leukocyte extravasation, with respect to their intrathymic location and temporal fluctuations. We find that CD34, MECA79, VCAM-1, ICAM-1 and VAP-1 are all expressed in thymic blood vessels. MECA79 and VAP-1 appear to be specific for post-capillary venules, while ICAM-1 and VCAM-1 are also found on intrathymic stromal cells. MAdCAM is also expressed in the thymus, but is not associated with vascular tissues. Only MECA79 is upregulated during recruitment peaks, suggesting a role for this molecule in the periodicity of recruitment. Together, these studies reveal potential roles for L-selectin ligands, VCAM-1, ICAM-1 and VAP-1 in progenitor recruitment to the thymus, and implicate the presence of other periodic signals, such as chemokines and cytokines, that cooperate to execute this essential function. |
| Keywords: | immunohistochemistry; protein expression; unclassified drug; nonhuman; t lymphocyte; t-lymphocytes; animal cell; mouse; animals; mice; animal tissue; cd34 antigen; bone marrow; vascular cell adhesion molecule 1; animal model; membrane proteins; mice, inbred c57bl; stem cell; lymphocyte differentiation; cytokine; chemokine; antigen; thymus; thymus gland; hematopoietic stem cells; cell migration; cell adhesion molecules; venules; blood flow; stromal cells; intercellular adhesion molecule 1; antigens, surface; immunoglobulins; intercellular adhesion molecule-1; perinatal period; adhesion; vascular cell adhesion molecule-1; l selectin; homing receptor; extravasation; vascular adhesion protein 1; periodicity; male; priority journal; article; progenitor homing; meca79 antigen; reflex recruitment; amine oxidase (copper-containing); mucoproteins |
| Journal Title: | Clinical and Developmental Immunology |
| Volume: | 10 |
| Issue: | 1 |
| ISSN: | 1740-2522 |
| Publisher: | Hindawi Publishing Corporation |
| Date Published: | 2003-03-01 |
| Start Page: | 27 |
| End Page: | 33 |
| Language: | English |
| DOI: | 10.1080/10446670310001598492 |
| PUBMED: | 14575155 |
| PROVIDER: | scopus |
| PMCID: | PMC2270677 |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Source: Scopus |
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