A stereotactic method for the three-dimensional registration of multi-modality biologic images in animals: NMR, PET, histology, and autoradiography Journal Article

Authors: Humm, J. L.; Ballon, D.; Hu, Y. C.; Ruan, S.; Chui, C.; Tulipano, P. K.; Erdi, A.; Koutcher, J.; Zakian, K.; Urano, M.; Zanzonico, P.; Mattis, C.; Dyke, J.; Chen, Y.; Harrington, P.; O'Donoghue, J. A.; Ling, C. C.
Article Title: A stereotactic method for the three-dimensional registration of multi-modality biologic images in animals: NMR, PET, histology, and autoradiography
Abstract: The objective of this work was to develop and then validate a stereotactic fiduciary marker system for tumor xenografts in rodents which could be used to co-register magnetic resonance imaging (MRI), PET, tissue histology, autoradiography, and measurements from physiologic probes. A Teflon™ fiduciary template has been designed which allows the precise insertion of small hollow Teflon rods (0.71 mm diameter) into a tumor. These rods can be visualized by MRI and PET as well as by histology and autoradiography on tissue sections. The methodology has been applied and tested on a rigid phantom, on tissue phantom material, and finally on tumor bearing mice. Image registration has been performed between the MRI and PET images for the rigid Teflon phantom and among MRI, digitized microscopy images of tissue histology, and autoradiograms for both tissue phantom and tumor-bearing mice. A registration accuracy, expressed as the average Euclidean distance between the centers of three fiduciary markers among the registered image sets, of 0.2 ± 0.06 mm was achieved between MRI and microPET image sets of a rigid Teflon phantom. The fiduciary template allows digitized tissue sections to be co-registered with three-dimensional MRI images with an average accuracy of 0.21 and 0.25 mm for the tissue phantoms and tumor xenografts, respectively. Between histology and autoradiograms, it was 0.19 and 0.21 mm for tissue phantoms and tumor xenografts, respectively. The fiduciary marker system provides a coordinate system with which to correlate information from multiple image types, on a voxel-by-voxel basis, with sub-millimeter accuracy-even among imaging modalities with widely disparate spatial resolution and in the absence of identifiable anatomic landmarks. © 2003 American Association of Physicists in Medicine.
Keywords: controlled study; middle aged; microscopy; carcinoma, squamous cell; nonhuman; validation process; positron emission tomography; magnetic resonance imaging; sensitivity and specificity; accuracy; reproducibility of results; mouse; animals; mice; animal tissue; image analysis; image interpretation, computer-assisted; animal experiment; animal model; tumor xenograft; algorithms; histology; animalia; image enhancement; subtraction technique; imaging, three-dimensional; measurement; magnetic resonance spectroscopy; intermethod comparison; rodentia; phantoms, imaging; signal processing, computer-assisted; nuclear magnetic resonance; angiography; autoradiography; image reconstruction; image registration; tomography, emission-computed; humans; male; priority journal; article; insertion sequences; fiduciary markers; rodent imaging; photogrammetry
Journal Title: Medical Physics
Volume: 30
Issue: 9
ISSN: 0094-2405
Publisher: American Association of Physicists in Medicine  
Date Published: 2003-09-01
Start Page: 2303
End Page: 2314
Language: English
DOI: 10.1118/1.1600738
PUBMED: 14528951
PROVIDER: scopus
Notes: Export Date: 12 September 2014 -- Source: Scopus
Altmetric Score
MSK Authors
  1. Yuchun Chen
    7 Chen
  2. Douglas J Ballon
    33 Ballon
  3. John Laurence Humm
    342 Humm
  4. Pat B Zanzonico
    246 Zanzonico
  5. Kristen L Zakian
    74 Zakian
  6. Jason A Koutcher
    236 Koutcher
  7. Shutian Ruan
    44 Ruan
  8. C Clifton Ling
    313 Ling
  9. Moneyasu Urano
    23 Urano
  10. Yu-Chi Hu
    60 Hu
  11. Chen Chui
    113 Chui
  12. Jonathan   Dyke
    10 Dyke
  13. Alev K Erdi
    12 Erdi