Image deconvolution in digital autoradiography: A preliminary study Journal Article
| Authors: | Zhang, M.; Chen, Q.; Li, X. F. ; O'Donoghue, J.; Ruan, S.; Zanzonico, P.; Ling, C. C.; Humm, J. L. |
| Article Title: | Image deconvolution in digital autoradiography: A preliminary study |
| Abstract: | Digital autoradiography (DAR) is a powerful method to determine quantitatively the "small-scale" (i.e., submillimeter) distribution of a radiotracer within a tissue section. However, the limited spatial resolution of the DAR image, due to blurring by the point spread function (PSF), can result in a poor correlation with tissue histology and immunohistochemistry. The authors attempt to overcome this limitation by recovering the radiotracer distribution by image deconvolution using the Richardson-Lucy algorithm and a measured PSF obtained from a small radioactive source on hydrophobic microscope slide. Simulation studies have shown that the deconvolution algorithm reliably recovers the pixel values corresponding to the radioactivity distributions. As an example, the proposed image restoration approach has been tested with DAR images of different radiolabeled markers on tumor sections obtained from clinical and preclinical animal model studies. Digital autoradiograms following deconvolution show improved sharpness and contrast relative to the unprocessed autoradiograms. © 2008 American Association of Physicists in Medicine. |
| Keywords: | controlled study; human tissue; unclassified drug; human cell; microscopy; nonhuman; positron emission tomography; sensitivity and specificity; neoplasms; reproducibility of results; animals; image interpretation, computer-assisted; animal experiment; animal model; algorithms; animalia; simulation; tissue section; isotope labeling; algorithm; image enhancement; pilot projects; contrast enhancement; image quality; rat; radioactivity; reliability; iodine 124; tracer; phantoms, imaging; tumor; image processing; signal processing, computer-assisted; artifacts; autoradiography; image reconstruction; digital imaging; radiographic image enhancement; hydrophobicity; iodoazomycin galactopyranoside i 124; digital autoradiography; image deconvolution; imaging plate; point spread function; richardson-lucy algorithm; 5 nitroimidazole derivative |
| Journal Title: | Medical Physics |
| Volume: | 35 |
| Issue: | 2 |
| ISSN: | 0094-2405 |
| Publisher: | American Association of Physicists in Medicine |
| Date Published: | 2008-01-01 |
| Start Page: | 522 |
| End Page: | 530 |
| Language: | English |
| DOI: | 10.1118/1.2828198 |
| PUBMED: | 18383673 |
| PROVIDER: | scopus |
| PMCID: | PMC2668928 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 2" - "Export Date: 17 November 2011" - "CODEN: MPHYA" - "Source: Scopus" |
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