ALC (adjacent to LMX1 in chick) is a novel dorsal limb mesenchyme marker Journal Article
| Authors: | Holmes, G.; Crooijmans, R.; Groenen, M.; Niswander, L. |
| Article Title: | ALC (adjacent to LMX1 in chick) is a novel dorsal limb mesenchyme marker |
| Abstract: | During dorsal-ventral (DV) patterning of the vertebrate limb, WNT7A is expressed in dorsal limb ectoderm and activates the expression of LMX1 (in chick; Lmx1b in mouse) in dorsal limb mesenchyme, resulting in the appropriate development of dorsal cell fates. These two genes are the only known factors involved in directing dorsal patterning and the molecular events that link these two factors or that occur downstream of LMX1/1b are unknown. We have isolated a novel chick transcript, ALC (adjacent to LMX1 in chick). ALC is located 5.3 kb from the 5′ end of LMX1 and is transcribed in the opposite direction. It is expressed in a sub-set of tissues expressing LMX1, most notably in the dorsal mesenchyme of the limb, and thus is the second gene discovered with such a distribution in the limb. Misexpression studies with viral constructs show that ALC is downstream of WNT7A but not of LMX1. ALC shows no homology to known genes and its function remains to be determined. However, similarly placed transcripts occur in the human and mouse genomes, and we demonstrate that a mouse transcript is also expressed in dorsal limb mesenchyme. © 2003 Elsevier B.V. All rights reserved. |
| Keywords: | signal transduction; controlled study; unclassified drug; nonhuman; biological markers; animal cell; mouse; animals; mice; animal tissue; gene expression; embryo; transcription factor; embryo pattern formation; gene function; genetic transcription; homeodomain proteins; vertebrata; cloning, molecular; molecular sequence data; limb; rna, messenger; human genome; nucleotide sequence; membrane protein; extremities; base sequence; dna flanking region; sequence homology; wnt protein; mesoderm; genetic marker; mesenchyme; gene location; chromosome mapping; gene construct; chick embryo; chickens; patterning; ectoderm; limb development; virus gene; chick; limb bud; gene isolation; dorsal; human; article; gene components; alc; lmx1; lmx1b; wnt7a; protein lmx1; transcription factor alc; comparative gene mapping |
| Journal Title: | Gene Expression Patterns |
| Volume: | 3 |
| Issue: | 6 |
| ISSN: | 1567-133X |
| Publisher: | Elsevier B.V. |
| Date Published: | 2003-12-01 |
| Start Page: | 735 |
| End Page: | 741 |
| Language: | English |
| DOI: | 10.1016/s1567-133x(03)00139-x |
| PUBMED: | 14643681 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Source: Scopus |
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