A simple method for detecting up to five immunofluorescent parameters together with DNA staining for cell cycle or viability on a benchtop flow cytometer Journal Article

  

Authors:	Gordon, K. M.; Duckett, L.; Daul, B.; Petrie, H. T.
Article Title:	A simple method for detecting up to five immunofluorescent parameters together with DNA staining for cell cycle or viability on a benchtop flow cytometer
Abstract:	In this manuscript, we describe modifications to a commercial three-laser benchtop flow cytometer, as well as relevant biological methods, that allow analysis of up to five immunofluorescent parameters together with an ultraviolet (UV)-excitable DNA stain. This method allows expanded capacity for multiparameter immunophenotyping of complex mixed cell populations, together with accurate measurements of DNA content (cell cycle) or cell viability, on a stable, end-user operated platform. © 2003 Elsevier Science B.V. All rights reserved.
Keywords:	controlled study; nonhuman; flow cytometry; ultraviolet radiation; accuracy; animal cell; mouse; cell viability; cell survival; cell cycle; biological model; analytic method; fluorescent dyes; immunofluorescence; cell population; fluorescent antibody technique; dna; lasers; staining; data analysis; measurement; immunophenotyping; indoles; parameter; staining and labeling; chemical modification; dna content; cell cycle analysis; priority journal; article; dna staining; propidium
Journal Title:	Journal of Immunological Methods
Volume:	275
Issue:	1-2
ISSN:	0022-1759
Publisher:	Elsevier Science, Inc.
Date Published:	2003-04-01
Start Page:	113
End Page:	121
Language:	English
DOI:	10.1016/s0022-1759(03)00009-7
PUBMED:	12667675
PROVIDER:	scopus
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-0344177194&partnerID=40&md5=4759bf3cbbee5b8622e0f77b705fcfb6
Notes:	Export Date: 12 September 2014 -- Source: Scopus

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