Ku86 deficiency leads to reduced intrachromosomal homologous recombination in vivo in mice Journal Article
| Authors: | Reliene, R.; Bishop, A. J. R.; Li, G.; Schiestl, R. H. |
| Article Title: | Ku86 deficiency leads to reduced intrachromosomal homologous recombination in vivo in mice |
| Abstract: | Ku70 and Ku86 together with DNA-PKcs form the DNA-dependent protein kinase (DNA-PK) complex that is involved in DNA double-strand break repair by nonhomologous end joining. We investigated the effect of Ku86 mutation on intrachromosomal homologous recombination (HR) resulting in deletions in vivo in mice. We quantified such deletion events using a phenotypic pigmentation assay. Deletion of one copy of a 70kb DNA duplication in the pink-eyed unstable (pun) allele results in reversion to the wildtype pink-eyed dilution (p) gene, allowing black pigment accumulation in cells of the retinal pigment epithelium (RPE). We found that the frequency of homologous recombination was significantly reduced in Ku86 deficient mice. Furthermore, the proliferation of cells in which recombination events occurred was reduced and developmentally delayed in the Ku86 deficient mice. These data indicate a role for Ku86 directly or indirectly in homologous recombination in vivo. © 2003 Elsevier B.V. All rights reserved. |
| Keywords: | controlled study; unclassified drug; gene mutation; gene deletion; dna-binding proteins; sequence deletion; nonhuman; cell proliferation; animal cell; mouse; phenotype; animals; mice; mice, knockout; animal tissue; in vivo study; mice, inbred c57bl; dna; genetic recombination; recombination, genetic; quantitative analysis; data analysis; gene duplication; protein deficiency; ku antigen; chromosomes; in vivo; genetic instability; pigment epithelium; eye color; antigens, nuclear; dilution; priority journal; article; ku86; pun reversions; protein ku86; pigment epithelium of eye |
| Journal Title: | DNA Repair |
| Volume: | 3 |
| Issue: | 2 |
| ISSN: | 1568-7864 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2004-02-01 |
| Start Page: | 103 |
| End Page: | 111 |
| Language: | English |
| DOI: | 10.1016/j.dnarep.2003.09.010 |
| PROVIDER: | scopus |
| PUBMED: | 14706343 |
| DOI/URL: | |
| Notes: | DNA Repair -- Cited By (since 1996):9 -- Export Date: 16 June 2014 -- CODEN: DRNEA -- Source: Scopus |
