Ku70 is required for DNA repair but not for T cell antigen receptor gene recombination in vivo Journal Article
| Authors: | Ouyang, H.; Nussenzweig, A.; Kurimasa, A.; Soares, V. D. C.; Li, X.; Cordon-Cardo, C.; Li, W. H.; Cheong, N.; Nussenzweig, M.; Iliakis, G.; Chen, D. J.; Li, G. C. |
| Article Title: | Ku70 is required for DNA repair but not for T cell antigen receptor gene recombination in vivo |
| Abstract: | Ku is a complex of two proteins, Ku70 and Ku80, and functions as a heterodimer to bind DNA double-strand breaks (DSB) and activate DNA- dependent protein kinase. The role of the Ku70 subunit in DNA DSB repair, hypersensitivity to ionizing radiation, and V(D)J recombination was examined in mice that lack Ku70 (Ku70(-/-)). Like Ku80(-/-) mice, Ku70(-/-) mice showed a profound deficiency in DNA DSB repair and were proportional dwarfs. Surprisingly, in contrast to Ku80(-/-) mice in which both T and B lymphocyte development were arrested at an early stage, lack of Ku70 was compatible with T cell receptor gene recombination and the development of mature CD4+CD8- and CD4-CD8+ T cells. Our data shows, for the first time, that Ku70 plays an essential role in DNA DSB repair, but is not required for TCR V(D)J recombination. These results suggest that distinct but overlapping repair pathways may mediate DNA DSB repair and V(D)J recombination. |
| Keywords: | controlled study; dna-binding proteins; nonhuman; polymerase chain reaction; t lymphocyte; t-lymphocytes; protein analysis; animal cell; mouse; animals; mice; mice, knockout; animal tissue; gene targeting; dna repair; cell differentiation; b lymphocyte; b-lymphocytes; nuclear proteins; lymphocyte differentiation; t lymphocyte receptor; dna; genetic recombination; molecular sequence data; receptors, antigen, t-cell; gene disruption; recombination, genetic; ionizing radiation; base sequence; dna primers; protein subunit; dna helicases; gene rearrangement, t-lymphocyte; antigens, nuclear; priority journal; article |
| Journal Title: | Journal of Experimental Medicine |
| Volume: | 186 |
| Issue: | 6 |
| ISSN: | 0022-1007 |
| Publisher: | Rockefeller University Press |
| Date Published: | 1997-09-15 |
| Start Page: | 921 |
| End Page: | 929 |
| Language: | English |
| DOI: | 10.1084/jem.186.6.921 |
| PUBMED: | 9294146 |
| PROVIDER: | scopus |
| PMCID: | PMC2199057 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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132Li -
16
Ouyang -
612
Cordon-Cardo -
6
Li -
18
Nussenzweig -
22Soares
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