SetB: An integral membrane protein that affects chromosome segregation in Escherichia coli Journal Article

  

Authors:	Espeli, O.; Nurse, P.; Levine, C.; Lee, C.; Marians, K. J.
Article Title:	SetB: An integral membrane protein that affects chromosome segregation in Escherichia coli
Abstract:	setB was identified as a high-copy suppressor of the partition defect of a mutation in parC, encoding one of the subunits of topoisomerase IV. Deletion of this integral inner membrane protein causes a delay in chromosome segregation, whereas its overproduction causes nucleoid disintegration and stretching, leading to a cell division defect. setB deletion mutants also exhibit a synthetic phenotype when combined with mutations that delete the C-terminal motor domain of the septal ring protein FtsK. SetB localizes in the cell as a helix and interacts with MreB, the bacterial actin homologue, which also forms a helix. These observations suggest that there may be a link between chromosome segregation and cellular infrastructure.
Keywords:	controlled study; unclassified drug; gene mutation; sequence analysis; gene deletion; nonhuman; protein localization; phenotype; actin; cell division; carboxy terminal sequence; protein protein interaction; protein; membrane proteins; bacteria (microorganisms); protein synthesis; escherichia coli; membrane protein; cellular distribution; protein subunit; chromosome analysis; dna topoisomerase iv; mreb protein; chromosome segregation; chromosomes, bacterial; escherichia coli proteins; protein ftsk; negibacteria; monosaccharide transport proteins; priority journal; article; protein setb
Journal Title:	Molecular Microbiology
Volume:	50
Issue:	2
ISSN:	0950-382X
Publisher:	Blackwell Publishing
Date Published:	2003-10-01
Start Page:	495
End Page:	509
Language:	English
DOI:	10.1046/j.1365-2958.2003.03736.x
PUBMED:	14617174
PROVIDER:	scopus
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-0142093602&partnerID=40&md5=f026b3b29a8b922bb94d203ed6dff7ea
Notes:	Export Date: 12 September 2014 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/59579