Monitoring CD4+ T cell responses against viral and tumor antigens using T cells as novel target APC Journal Article


Authors: Atanackovic, D.; Matsuo, M.; Ritter, E.; Mazzara, G.; Ritter, G.; Jäger, E.; Knuth, A.; Old, L. J.; Gnjatic, S.
Article Title: Monitoring CD4+ T cell responses against viral and tumor antigens using T cells as novel target APC
Abstract: CD4+ T cells play an important role in the induction and maintenance of an effective antiviral and antitumor immune response. However, standardized monitoring of antigen-specific CD4+ T cells has not been established at the single-cell level. We now present a sensitive, specific, and simple methodology in which purified memory CD4+ T cells are expanded from PBMC in a single cycle of antigen-driven stimulation and quantitatively assayed by interferon-γ ELISPOT. Issues of nonspecific background in assays were resolved with the use of innovative target cells, autologous PHA-expanded CD4+ T cells (T-APC). Remarkably, T-APC could not only present peptide epitopes from model antigens NY-ESO-1 and influenza nucleoprotein, but could also process full-length antigen endogenously expressed from recombinant fowlpox vector. This approach makes it possible to monitor CD4+ T cells in large series of patients, regardless of HLA haplotype, against the full peptide repertoire of a given antigen. © 2003 Elsevier B.V. All rights reserved.
Keywords: controlled study; human cell; nonhuman; antigen expression; t lymphocyte; cd8-positive t-lymphocytes; animals; melanoma; carcinoma, non-small-cell lung; lung neoplasms; peptide; tumor antigen; antigen presentation; lymphocyte activation; mononuclear cell; antigens, neoplasm; gamma interferon; quantitative analysis; immunomagnetic separation; hla dr antigen; major histocompatibility antigen class 2; cd4-positive t-lymphocytes; epitope; virus antigen; immunoassay; immunostimulation; t-lymphocyte subsets; enzyme linked immunospot assay; memory cell; antigen-presenting cells; virus protein; virus vector; expression vector; antigens, viral; cd4+ t cell; fowlpox virus; nucleoprotein; humans; human; priority journal; article; antigen presentation and processing; immuno-monitoring; viral and tumor antigen; immunological technique
Journal Title: Journal of Immunological Methods
Volume: 278
Issue: 1-2
ISSN: 0022-1759
Publisher: Elsevier Science, Inc.  
Date Published: 2003-01-01
Start Page: 57
End Page: 66
Language: English
DOI: 10.1016/s0022-1759(03)00209-6
PUBMED: 12957396
PROVIDER: scopus
DOI/URL:
Notes: Export Date: 12 September 2014 -- Source: Scopus
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MSK Authors
  1. Mitsutoshi Matsuo
    9 Matsuo
  2. Sacha Gnjatic
    113 Gnjatic
  3. Gerd Ritter
    166 Ritter
  4. Erika Ritter
    37 Ritter
  5. Lloyd J Old
    593 Old