Substrate specificity of NovM: Implications for novobiocin biosynthesis and glycorandomization Journal Article
| Authors: | Albermann, C.; Soriano, A.; Jiang, J.; Vollmer, H.; Biggins, J. B.; Barton, W. A.; Lesniak, J.; Nikolov, D. B.; Thorson, J. S. |
| Article Title: | Substrate specificity of NovM: Implications for novobiocin biosynthesis and glycorandomization |
| Abstract: | (Matrix presented) In an effort to expand the scope of natural product in vitro glycorandomization (IVG), the substrate specificity of NovM was investigated. A test of four aglycon analogues and over 40 nucleotide sugars revealed NovM has a surprisingly stringent substrate specificity and provided only three new "unnatural" natural products. On the basis of the determined substrate specificity, an alternative to the sugar nucleotide biosynthetic dogma and a cautionary note for the general applicability of IVG are introduced. |
| Keywords: | unclassified drug; nonhuman; biosynthesis; antiinfective agent; chemistry; anti-bacterial agents; substrate specificity; randomization; natural product; enzyme specificity; nucleotide; glycosides; sugar; glycoside; drug purification; streptomyces; novobiocin; article; novobiocin m |
| Journal Title: | Organic Letters |
| Volume: | 5 |
| Issue: | 6 |
| ISSN: | 1523-7060 |
| Publisher: | American Chemical Society |
| Date Published: | 2003-03-20 |
| Start Page: | 933 |
| End Page: | 936 |
| Language: | English |
| DOI: | 10.1021/ol0341086 |
| PUBMED: | 12633109 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 12 September 2014 -- Source: Scopus |
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