Correlation of in vivo and in vitro measures of carbonic anhydrase IX antigen expression in renal masses using antibody (124)I-cG250 Journal Article
| Authors: | Pryma, D. A.; O'Donoghue, J. A.; Humm, J. L.; Jungbluth, A. A.; Old, L. J.; Larson, S. M.; Divgi, C. R. |
| Article Title: | Correlation of in vivo and in vitro measures of carbonic anhydrase IX antigen expression in renal masses using antibody (124)I-cG250 |
| Abstract: | The goal of this study was to determine whether there is a potential correlation between quantification of radiolabeled macromolecular uptake in tumors determined in vivo using PET/CT and in vitro using autoradiography and γ-counting of tumor tissue. Methods: Twenty-six patients with renal masses scheduled for surgical resection received 124I-labeled antibody cG250. Tumor specimens obtained from resection were studied. Fifteen of these patients had clear cell cancer demonstrated by positive findings on PET/CT images and histopathology. Radioactivity in tumors was measured on PET/CT images and expressed as percentage injected dose per gram. These values were then normalized to measurements of known serum radioactivity from a venous blood sample obtained at the time of PET/CT. Comparable measurements were obtained in vitro using γ-well counting and digital autoradiography of tumor tissue. Results: There was a significant correlation between tumor radioactivity estimated in vivo and in vitro (Spearman correlation coefficient comparing normalized PET measurements with well counting of 0.84, P<0.000001, and with autoradiography of 0.88, P<0.000001). PET/CT measurements of tumor uptake were lower than measurements obtained with either of the in vitro methods, and digital autoradiography resulted in the highest measurements. Conclusion: PET/CT can be reliably used to quantify radiolabeled macromolecular uptake in vivo, suggesting important implications for quantitative pharmacokinetic estimates of macromolecular biodistribution. Copyright © 2011 the American Physiological Society. |
| Keywords: | clinical article; controlled study; human tissue; unclassified drug; cancer diagnosis; antigen expression; tumor biopsy; in vivo study; in vitro study; molecular imaging; correlation analysis; carbonate dehydratase ix; drug distribution; kidney tumor; pilot study; radioactivity; computer assisted emission tomography; open study; kidney cancer; autoradiography; antibody labeling; renal cancer; venous blood; 124i-cg250; immunope.t.; cg250; girentuximab i 124 |
| Journal Title: | Journal of Nuclear Medicine |
| Volume: | 52 |
| Issue: | 4 |
| ISSN: | 0161-5505 |
| Publisher: | Society of Nuclear Medicine |
| Date Published: | 2011-04-01 |
| Start Page: | 535 |
| End Page: | 540 |
| Language: | English |
| DOI: | 10.2967/jnumed.110.083295 |
| PROVIDER: | scopus |
| PUBMED: | 21421715 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 23 June 2011" - "CODEN: JNMEA" - "Source: Scopus" |
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151O'Donoghue -
439Humm -
459Jungbluth -
962Larson -
593Old
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