Technical note: Scintillation well counters and particle counting digital autoradiography devices can be used to detect activities associated with genomic profiling adequacy of biopsy specimens obtained after a low activity (18)F-FDG injection Journal Article

   

Authors:	Kirov, A. S.; Fanchon, L. M.; Seiter, D.; Czmielewski, C.; Russell, J.; Dogan, S.; Carlin, S.; Pinker-Domenig, K.; Yorke, E.; Schmidtlein, C. R.; Boyko, V.; Fujisawa, S.; Manova-Todorova, K.; Zanzonico, P.; Dauer, L.; Deasy, J. O.; Humm, J. L.; Solomon, S.
Article Title:	Technical note: Scintillation well counters and particle counting digital autoradiography devices can be used to detect activities associated with genomic profiling adequacy of biopsy specimens obtained after a low activity (18)F-FDG injection
Abstract:	Purpose: Genomic profiling of biopsied tissue is the basis for precision cancer therapy. However, biopsied materials may not contain sufficient amounts of tumor deoxyribonucleonic acid needed for the analysis. We propose a method to determine the adequacy of specimens for performing genomic profiling by quantifying their metabolic activity. Methods: We estimated the average density of tumor cells in biopsy specimens needed to successfully perform genomic analysis following the Memorial Sloan Kettering Integrated Mutation Profiling of Actionable Cancer Targets (MSK-IMPACT) protocol from the minimum amount of deoxyribonucleonic acid needed and the volume of tissue typically used for analysis. The average 18F-FDG uptake per cell was assessed by incubating HT-29 adenocarcinoma tumor cells in 18F-FDG containing solution and then measuring their activity with a scintillation well counter. Consequently, we evaluated the response of two devices around the minimum expected activities which would indicate genomic profiling adequacy of biopsy specimens obtained under 18F-FDG PET/CT guidance. Surrogate samples obtained using 18G core needle biopsies of gels containing either 18F-FDG-loaded cells in the expected concentrations or the corresponding activity were measured using autoradiography and a scintillation well counter. Autoradiography was performed using a CCD-based device with real-time image display as well as with digital autoradiography imaging plates following a 30-min off-line protocol for specimen activity determination against previously established calibration. Results: Cell incubation experiments and estimates obtained from quantitative autoradiography of biopsy specimens (QABS) indicate that specimens acquired under 18F-FDG PET/CT guidance that contained the minimum amount of cells needed for genomic profiling would have an average activity concentration in the range of about 3 to about 9 kBq/mL. When exposed to specimens with similar activity concentration, both a CCD-based autoradiography device and a scintillation well counter produced signals with sufficient signal-to-background ratio for specimen genomic adequacy identification in less than 10 min, which is short enough to allow procedure guidance. Conclusion: Scintillation well counter measurements and CCD-based autoradiography have adequate sensitivity to detect the tumor burden needed for genomic profiling during 18F-FDG PET/CT-guided 18G core needle biopsies of liver adenocarcinoma metastases. © 2018 American Association of Physicists in Medicine
Keywords:	biopsy; autoradiography; beta particles; fdg pet; scintillation cameras
Journal Title:	Medical Physics
Volume:	45
Issue:	5
ISSN:	0094-2405
Publisher:	American Association of Physicists in Medicine
Date Published:	2018-05-01
Start Page:	2179
End Page:	2185
Language:	English
DOI:	10.1002/mp.12836
PROVIDER:	scopus
PMCID:	PMC5948146
PUBMED:	29480927
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85044185168&doi=10.1002%2fmp.12836&partnerID=40&md5=e26d981c2e216225ab5eec05ec08bbc2
Notes:	Article -- Export Date: 1 June 2018 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/125115