Technical note: Scintillation well counters and particle counting digital autoradiography devices can be used to detect activities associated with genomic profiling adequacy of biopsy specimens obtained after a low activity (18)F-FDG injection Journal Article


Authors: Kirov, A. S.; Fanchon, L. M.; Seiter, D.; Czmielewski, C.; Russell, J.; Dogan, S.; Carlin, S.; Pinker-Domenig, K.; Yorke, E.; Schmidtlein, C. R.; Boyko, V.; Fujisawa, S.; Manova-Todorova, K.; Zanzonico, P.; Dauer, L.; Deasy, J. O.; Humm, J. L.; Solomon, S.
Article Title: Technical note: Scintillation well counters and particle counting digital autoradiography devices can be used to detect activities associated with genomic profiling adequacy of biopsy specimens obtained after a low activity (18)F-FDG injection
Abstract: Purpose: Genomic profiling of biopsied tissue is the basis for precision cancer therapy. However, biopsied materials may not contain sufficient amounts of tumor deoxyribonucleonic acid needed for the analysis. We propose a method to determine the adequacy of specimens for performing genomic profiling by quantifying their metabolic activity. Methods: We estimated the average density of tumor cells in biopsy specimens needed to successfully perform genomic analysis following the Memorial Sloan Kettering Integrated Mutation Profiling of Actionable Cancer Targets (MSK-IMPACT) protocol from the minimum amount of deoxyribonucleonic acid needed and the volume of tissue typically used for analysis. The average 18F-FDG uptake per cell was assessed by incubating HT-29 adenocarcinoma tumor cells in 18F-FDG containing solution and then measuring their activity with a scintillation well counter. Consequently, we evaluated the response of two devices around the minimum expected activities which would indicate genomic profiling adequacy of biopsy specimens obtained under 18F-FDG PET/CT guidance. Surrogate samples obtained using 18G core needle biopsies of gels containing either 18F-FDG-loaded cells in the expected concentrations or the corresponding activity were measured using autoradiography and a scintillation well counter. Autoradiography was performed using a CCD-based device with real-time image display as well as with digital autoradiography imaging plates following a 30-min off-line protocol for specimen activity determination against previously established calibration. Results: Cell incubation experiments and estimates obtained from quantitative autoradiography of biopsy specimens (QABS) indicate that specimens acquired under 18F-FDG PET/CT guidance that contained the minimum amount of cells needed for genomic profiling would have an average activity concentration in the range of about 3 to about 9 kBq/mL. When exposed to specimens with similar activity concentration, both a CCD-based autoradiography device and a scintillation well counter produced signals with sufficient signal-to-background ratio for specimen genomic adequacy identification in less than 10 min, which is short enough to allow procedure guidance. Conclusion: Scintillation well counter measurements and CCD-based autoradiography have adequate sensitivity to detect the tumor burden needed for genomic profiling during 18F-FDG PET/CT-guided 18G core needle biopsies of liver adenocarcinoma metastases. © 2018 American Association of Physicists in Medicine
Keywords: biopsy; autoradiography; beta particles; fdg pet; scintillation cameras
Journal Title: Medical Physics
Volume: 45
Issue: 5
ISSN: 0094-2405
Publisher: American Association of Physicists in Medicine  
Date Published: 2018-05-01
Start Page: 2179
End Page: 2185
Language: English
DOI: 10.1002/mp.12836
PROVIDER: scopus
PMCID: PMC5948146
PUBMED: 29480927
DOI/URL:
Notes: Article -- Export Date: 1 June 2018 -- Source: Scopus
Altmetric Score
MSK Authors
  1. Snjezana Dogan
    87 Dogan
  2. Stephen Solomon
    267 Solomon
  3. Ellen D Yorke
    314 Yorke
  4. John Laurence Humm
    342 Humm
  5. Pat B Zanzonico
    246 Zanzonico
  6. Lawrence Dauer
    101 Dauer
  7. James Russell
    29 Russell
  8. Assen Kirov
    60 Kirov
  9. Joseph Owen Deasy
    279 Deasy
  10. Louise Marianne Fanchon
    10 Fanchon
  11. Vitaly P Boyko
    4 Boyko