Neutrophil IL-10 suppresses peritoneal inflammatory monocytes during polymicrobial sepsis Journal Article
| Authors: | Ocuin, L. M.; Bamboat, Z. M.; Balachandran, V. P.; Cavnar, M. J.; Obaid, H.; Plitas, G.; DeMatteo, R. P. |
| Article Title: | Neutrophil IL-10 suppresses peritoneal inflammatory monocytes during polymicrobial sepsis |
| Abstract: | Septic peritonitis remains a major cause of death. Neutrophils and inflammatory monocytes are principal components of the innate immune system and are essential for defense against a range of microbial pathogens. Their role and interaction in polymicrobial sepsis have not been defined clearly. Using a murine model of CLP to induce moderate sepsis, we found that neutrophil depletion did not alter survival, whereas depletion of neutrophils and inflammatory monocytes markedly reduced survival. After neutrophil depletion, inflammatory monocytes had greater phagocytic capacity and oxidative burst, and increased expression of costimulatory molecules, TNF, and iNOS. Notably, peritoneal neutrophils produced IL-10 following CLP. Adoptive i.p. transfer of WT but not IL-10-/- neutrophils into septic mice reduced monocyte expression of TNF. In vitro experiments confirmed that monocyte suppression was mediated by neutrophil-derived IL-10. Thus, during septic peritonitis, neutrophils suppress peritoneal inflammatory monocytes through IL-10 and are dispensable for survival. © Society for Leukocyte Biology. |
| Keywords: | survival; controlled study; protein expression; survival analysis; nonhuman; flow cytometry; animal cell; mouse; animals; mice; animal tissue; cell function; interleukin 10; animal experiment; animal model; inflammation; in vitro study; mice, inbred c57bl; cytokines; neutrophil; tumor necrosis factor-alpha; cell isolation; sepsis; innate immunity; neutrophils; monocyte; monocytes; phagocytosis; cell transfer; cell selection; tumor necrosis factor; inducible nitric oxide synthase; nitric oxide synthase type ii; interleukin-10; bacteria; cecal ligation and puncture; peritoneum cell; polymicrobial sepsis; peritonitis |
| Journal Title: | Journal of Leukocyte Biology |
| Volume: | 89 |
| Issue: | 3 |
| ISSN: | 0741-5400 |
| Publisher: | Federation of American Societies for Experimental Biology |
| Date Published: | 2011-03-01 |
| Start Page: | 423 |
| End Page: | 432 |
| Language: | English |
| DOI: | 10.1189/jlb.0810479 |
| PUBMED: | 21106642 |
| PROVIDER: | scopus |
| PMCID: | PMC3040467 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 23 June 2011" - "CODEN: JLBIE" - "Source: Scopus" |
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