Distinct properties of the XY pseudoautosomal region crucial for male meiosis Journal Article
| Authors: | Kauppi, L.; Barchi, M.; Baudat, F.; Romanienko, P. J.; Keeney, S.; Jasin, M. |
| Article Title: | Distinct properties of the XY pseudoautosomal region crucial for male meiosis |
| Abstract: | Meiosis requires that each chromosome find its homologous partner and undergo at least one crossover. X-Y chromosome segregation hinges on efficient crossing-over in a very small region of homology, the pseudoautosomal region (PAR). We find that mouse PAR DNA occupies unusually long chromosome axes, potentially as shorter chromatin loops, predicted to promote double-strand break (DSB) formation. Most PARs show delayed appearance of RAD51/DMC1 foci, which mark DSB ends, and all PARs undergo delayed DSB-mediated homologous pairing. Analysis of Spo11β isoform-specific transgenic mice revealed that late RAD51/DMC1 foci in the PAR are genetically distinct from both early PAR foci and global foci and that late PAR foci promote efficient X-Y pairing, recombination, and male fertility. Our findings uncover specific mechanisms that surmount the unique challenges of X-Y recombination. |
| Keywords: | controlled study; nonhuman; genetic analysis; animal cell; chromosome; chromosome structure; mouse; meiosis; animals; cell cycle proteins; mice; in situ hybridization, fluorescence; mice, inbred c57bl; transgenic mouse; mice, transgenic; dna strand breakage; nuclear proteins; genetic recombination; amino acid sequence; molecular sequence data; chromatin; dna breaks, double-stranded; x chromosome; base sequence; rodent; fertility; chromosome analysis; chromosome pairing; protein isoforms; chromosome segregation; chromatin structure; male fertility; endodeoxyribonucleases; recombination; crossing over, genetic; y chromosome; rad51 recombinase; homology; photosynthetically active radiation |
| Journal Title: | Science |
| Volume: | 331 |
| Issue: | 6019 |
| ISSN: | 0036-8075 |
| Publisher: | American Association for the Advancement of Science |
| Date Published: | 2011-02-18 |
| Start Page: | 916 |
| End Page: | 920 |
| Language: | English |
| DOI: | 10.1126/science.1195774 |
| PUBMED: | 21330546 |
| PROVIDER: | scopus |
| PMCID: | PMC3151169 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 2" - "Export Date: 23 June 2011" - "CODEN: SCIEA" - "Source: Scopus" |
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