Distinct properties of the XY pseudoautosomal region crucial for male meiosis Journal Article


Authors: Kauppi, L.; Barchi, M.; Baudat, F.; Romanienko, P. J.; Keeney, S.; Jasin, M.
Article Title: Distinct properties of the XY pseudoautosomal region crucial for male meiosis
Abstract: Meiosis requires that each chromosome find its homologous partner and undergo at least one crossover. X-Y chromosome segregation hinges on efficient crossing-over in a very small region of homology, the pseudoautosomal region (PAR). We find that mouse PAR DNA occupies unusually long chromosome axes, potentially as shorter chromatin loops, predicted to promote double-strand break (DSB) formation. Most PARs show delayed appearance of RAD51/DMC1 foci, which mark DSB ends, and all PARs undergo delayed DSB-mediated homologous pairing. Analysis of Spo11β isoform-specific transgenic mice revealed that late RAD51/DMC1 foci in the PAR are genetically distinct from both early PAR foci and global foci and that late PAR foci promote efficient X-Y pairing, recombination, and male fertility. Our findings uncover specific mechanisms that surmount the unique challenges of X-Y recombination.
Keywords: controlled study; nonhuman; genetic analysis; animal cell; chromosome; chromosome structure; mouse; meiosis; animals; cell cycle proteins; mice; in situ hybridization, fluorescence; mice, inbred c57bl; transgenic mouse; mice, transgenic; dna strand breakage; nuclear proteins; genetic recombination; amino acid sequence; molecular sequence data; chromatin; dna breaks, double-stranded; x chromosome; base sequence; rodent; fertility; chromosome analysis; chromosome pairing; protein isoforms; chromosome segregation; chromatin structure; male fertility; endodeoxyribonucleases; recombination; crossing over, genetic; y chromosome; rad51 recombinase; homology; photosynthetically active radiation
Journal Title: Science
Volume: 331
Issue: 6019
ISSN: 0036-8075
Publisher: American Association for the Advancement of Science  
Date Published: 2011-02-18
Start Page: 916
End Page: 920
Language: English
DOI: 10.1126/science.1195774
PUBMED: 21330546
PROVIDER: scopus
PMCID: PMC3151169
DOI/URL:
Notes: --- - "Cited By (since 1996): 2" - "Export Date: 23 June 2011" - "CODEN: SCIEA" - "Source: Scopus"
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MSK Authors
  1. Marco Barchi
    12 Barchi
  2. Scott N Keeney
    111 Keeney
  3. Frederic Baudat
    12 Baudat
  4. Liisa Hannele Kauppi
    10 Kauppi
  5. Maria Jasin
    223 Jasin