Single-lineage transcriptome analysis reveals key regulatory pathways in primitive erythroid progenitors in the mouse embryo Journal Article

   

Authors:	Isern, J.; He, Z.; Fraser, S. T.; Nowotschin, S.; Ferrer-Vaquer, A.; Moore, R.; Hadjantonakis, A. K.; Schulz, V.; Tuck, D.; Gallagher, P. G.; Baron, M. H.
Article Title:	Single-lineage transcriptome analysis reveals key regulatory pathways in primitive erythroid progenitors in the mouse embryo
Abstract:	Primitive erythroid (EryP) progenitors are the first cell type specified from the mesoderm late in gastrulation.We used a transgenic reporter to image and purify the earliest blood progenitors and their descendants from developing mouse embryos. EryP progenitors exhibited remarkable proliferative capacity in the yolk sac immediately before the onset of circulation, when these cells comprise nearly half of all cells of the embryo. Global expression profiles generated at 24-hour intervals from embryonic day 7.5 through 2.5 revealed 2 abrupt changes in transcript diversity that coincided with the entry of EryPs into the circulation and with their late maturation and enucleation, respectively. These changes were paralleled by the expression of critical regulatory factors. Experiments designed to test predictions from these data demonstrated that the Wnt-signaling pathway is active in EryP progenitors, which display an aerobic glycolytic profile and the numbers of which are regulated by transforming growth factor-β1 and hypoxia. This is the first transcriptome assembled for a single hematopoietic lineage of the embryo over the course of its differentiation. © 2011 by The American Society of Hematology.
Keywords:	signal transduction; nonhuman; cell proliferation; animal cell; gene expression; cell maturation; erythroid precursor cell; embryo development; reporter gene; transforming growth factor beta1; enucleation; wnt protein; transcriptome; yolk sac; embryo cell; circulation; anaerobic glycolysis
Journal Title:	Blood
Volume:	117
Issue:	18
ISSN:	0006-4971
Publisher:	American Society of Hematology
Date Published:	2011-05-05
Start Page:	4924
End Page:	4934
Language:	English
DOI:	10.1182/blood-2010-10-313676
PROVIDER:	scopus
PMCID:	PMC3100699
PUBMED:	21263157
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-79955969151&partnerID=40&md5=961136777820704622e37b526e6f9e13
Notes:	--- - "Cited By (since 1996): 1" - "Export Date: 23 June 2011" - "CODEN: BLOOA" - "Source: Scopus"

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