Early hematopoietic zinc finger protein (EHZF), the human homolog to mouse Evi3, is highly expressed in primitive human hematopoietic cells Journal Article
| Authors: | Bond, H. M.; Mesuraca, M.; Carbone, E.; Bonelli, P.; Agosti, V.; Amodio, N.; De Rosa, G.; Di Nicola, M.; Gianni, A. M.; Moore, M. A. S.; Hata, A.; Grieco, M.; Morrone, G.; Venuta, S. |
| Article Title: | Early hematopoietic zinc finger protein (EHZF), the human homolog to mouse Evi3, is highly expressed in primitive human hematopoietic cells |
| Abstract: | Comparison of the gene expression repertoire in human hematopoietic progenitors and mature leukocytes led to identification of a transcript expressed in CD34(+) cells and undetectable in differentiated cells. Sequencing of the cDNA (termed EHZF: early hematopoietic zinc finger) revealed 30 zinc fingers with 96% homology to mouse Evi3, a recently identified gene associated with the retroviral integration site in AKXD-27 B-cell lymphomas. EHZF and Evi3 share high homology with the transcription cofactor OAZ, implicated in the control of olfactory epithelium and B-lymphocyte differentiation and in the bone morphogenic protein (BMP) signal transduction. Here we show that (1) EHZF expression is abundant in human CD34+ progenitors and declines rapidly during cytokine-driven differentiation; (2) significant mRNA levels are found in most acute myelogenous leukemias; (3) in response to BMPs EHZF complexes SMADs 1 and 4, binds to, and enhances the transcriptional activity of, a BMP2/4 responsive element; (4) EHZF inhibits the transcriptional activity of early B-cell factor (EBF), a transcription factor essential for specification of the B-cell lineage. Taken together, our data suggest that EHZF is likely to play a relevant role in the control of human hematopoiesis and might be implicated in the development of hematopoietic malignancies. (C) 2004 by The American Society of Hematology. |
| Keywords: | transcription; in-vitro; differentiation; identification; gene-expression; stem-cells; bone-marrow; progenitors; integration; retroviral; stem/progenitor cells |
| Journal Title: | Blood |
| Volume: | 103 |
| Issue: | 6 |
| ISSN: | 0006-4971 |
| Publisher: | American Society of Hematology |
| Date Published: | 2004-03-15 |
| Start Page: | 2062 |
| End Page: | 2070 |
| Language: | English |
| ACCESSION: | WOS:000220123400021 |
| DOI: | 10.1182/blood-2003-07-2388 |
| PROVIDER: | wos |
| PUBMED: | 14630787 |
| Notes: | Article -- Source: Wos |
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