A rapid, direct, quantitative, and label-free detector of cardiac biomarker troponin T using near-infrared fluorescent single-walled carbon nanotube sensors Journal Article


Authors: Zhang, J.; Kruss, S.; Hilmer, A. J.; Shimizu, S.; Schmois, Z.; De La Cruz, F.; Barone, P. W.; Reuel, N. F.; Heller, D. A.; Strano, M. S.
Article Title: A rapid, direct, quantitative, and label-free detector of cardiac biomarker troponin T using near-infrared fluorescent single-walled carbon nanotube sensors
Abstract: Patients with chest pain account for 10% of US emergency room visits according to data from the Center for Disease Control and Prevention (2013). For triage of these patients, cardiac biomarkers troponin I and T are endorsed as standard indicators for acute myocardial infarction (AMI, or heart attack). Thus, there is significant interest in developing a rapid, point-of-care (POC) device for troponin detection. In this work, a rapid, quantitative, and label-free assay, which is specific for cardiac troponin T (cTnT) detection, using fluorescent single-walled carbon nanotubes (SWCNTs), is demonstrated. Chitosan-wrapped carbon nanotubes are cross-linked to form a thin gel that is further functionalized with nitrilotriacetic acid (NTA) moieties. Upon chelation of Ni2+, the Ni2+-NTA group binds to a hexa-histidine-modified troponin antibody, which specifically recognizes the target protein, troponin T. As the troponin T binds to the antibody, the local environment of the sensor changes, allowing direct troponin detection through intensity changes in SWCNT bandgap fluorescence. This platform represents the first near-infrared SWCNT sensor array for cTnT detection. Detection can be completed within 5 min, demonstrating a linear response to cTnT concentration and an experimental detection limit of 100 ng mL-1 (2.5 nm). This platform provides a promising new tool for POC AMI detection in the future. Moreover, the work presents two new methods of quantifying the number of amines and carboxylic groups, respectively, in a carbon hydrogel matrices. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Keywords: unclassified drug; human cell; protein analysis; protein targeting; fluorescence; amine; quantitative analysis; antigen binding; fluorescence analysis; antibody; nickel; chelation; carboxylic acid; cross linking; limit of detection; single walled nanotube; carbon nanotubes; near-infrared; troponin; troponin t; nanosensor; chitosan; nitrilotriacetic acid; human; priority journal; article; cardiac biomarkers; troponin antibody; near infrared imaging system; nickel chelation
Journal Title: Advanced Healthcare Materials
Volume: 3
Issue: 3
ISSN: 2192-2640
Publisher: Wiley V C H Verlag Gmbh  
Date Published: 2014-03-01
Start Page: 412
End Page: 423
Language: English
DOI: 10.1002/adhm.201300033
PROVIDER: scopus
PUBMED: 23966175
DOI/URL:
Notes: Export Date: 1 August 2014 -- Source: Scopus
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  1. Daniel Alan Heller
    73 Heller