Glutathione-S-transferase fusion protein nanosensor Research Letter
| Authors: | Williams, R. M.; Harvey, J. D.; Budhathoki-Uprety, J.; Heller, D. A. |
| Title: | Glutathione-S-transferase fusion protein nanosensor |
| Abstract: | Fusion protein tags are widely used to capture and track proteins in research and industrial bioreactor processes. Quantifying fusion-tagged proteins normally requires several purification steps coupled with classical protein assays. Here, we developed a broadly applicable nanosensor platform that quantifies glutathione-S-transferase (GST) fusion proteins in real-time. We synthesized a glutathione-DNA-carbon nanotube system to investigate glutathione-GST interactions via semiconducting single-walled carbon nanotube (SWCNT) photoluminescence. We found that SWCNT fluorescence wavelength and intensity modulation occurred specifically in response to GST and GST-fusions. The sensor response was dependent on SWCNT structure, wherein mod(n - m, 3) = 1 nanotube wavelength and intensity responses correlated with nanotube diameter distinctly from mod(n - m, 3) = 2 SWCNT responses. We also found broad functionality of this sensor to diverse GST-tagged proteins. This work comprises the first label-free optical sensor for GST and has implications for the assessment of protein expression in situ, including in imaging and industrial bioreactor settings. © 2020 American Chemical Society. |
| Keywords: | peptides; fusion proteins; bioreactors; protein expressions; nanotubes; single-walled carbon nanotubes (swcn); nanosensors; glutathione s-transferases; intensity modulations; industrial research; nanocarbon; singlewalled carbon nanotube (swcnt); affinity tag; fluorescent sensor; solvatochromism; fluorescence wavelength; nanotube diameters; sensor response |
| Journal Title: | Nano Letters |
| Volume: | 20 |
| Issue: | 10 |
| ISSN: | 1530-6984 |
| Publisher: | American Chemical Society |
| Date Published: | 2020-10-14 |
| Start Page: | 7287 |
| End Page: | 7295 |
| Language: | English |
| DOI: | 10.1021/acs.nanolett.0c02691 |
| PUBMED: | 32955895 |
| PROVIDER: | scopus |
| PMCID: | PMC8266418 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 December 2020 -- Source: Scopus |
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