Genetic variation in KLK2 and KLK3 is associated with concentrations of hK2 and PSA in serum and seminal plasma in young men Journal Article

Authors: Sävblom, C.; Halldén, C.; Cronin, A. M.; Säll, T.; Savage, C.; Vertosick, E. A.; Klein, R. J.; Giwercman, A.; Lilja, H.
Article Title: Genetic variation in KLK2 and KLK3 is associated with concentrations of hK2 and PSA in serum and seminal plasma in young men
Abstract: BACKGROUND: Genetic variants in KLK2 and KLK3 have been associated with increased serum concentrations of their encoded proteins, human kallikrein-related peptidase 2 (hK2) and prostate-specific antigen (PSA), and with prostate cancer in older men. Low PSA concentrations in seminal plasma (SP) have been associated with low sperm motility. To evaluate whether KLK2 and KLK3 genetic variants affect physiological prostatic secretion, we studied the association of SNPs with hK2 and PSA concentrations in SP and serum of young, healthy men. METHODS: Leukocyte DNA was extracted from 303 male military conscripts (median age 18.1 years). Nine SNPs across KLK2-KLK3 were genotyped. We measured PSA and hK2 in SP and serum using immunofluorometric assays. The association of genotype frequencies with hK2 and PSA concentrations was tested with the Kruskal-Wallis test. RESULTS: Four KLK2 SNPs (rs198972, rs198977, rs198978, and rs80050017) were strongly associated with hK2concentrations inSPand serum, with individuals homozygous for the major alleles having 3- to 7-fold higher concentrations than the intermediate concentrations found in other homozygotes and heterozygotes (all P < 0.001). Three of these SNPs were significantly associated with percentage of free PSA (%fPSA) in serum (all P < 0.007). Three KLK3 SNPs showed associations with PSA in SP, and the rs1058205 SNP was associated with total PSA in serum (P = 0.001) and %fPSA (P = 0.015). CONCLUSIONS: Associations observed in young, healthy men between the SP and serum concentrations of hK2 and PSA and several genetic variants in KLK2 and KLK3 could be useful to refine models of PSA cutoff values in prostate cancer testing. © 2013 American Association for Clinical Chemistry.
Keywords: adolescent; adult; young adult; unclassified drug; single nucleotide polymorphism; polymorphism, single nucleotide; prostate specific antigen; gene; genetic variability; genotype; gene frequency; heterozygote; prostate cancer; prostate-specific antigen; homozygosity; reference values; homozygote; dna extraction; serum; kallikreins; normal human; seminal plasma; spermatozoon motility; peptidase; tissue kallikreins; klk3 gene; semen; prostate secretion; genetic association studies; klk2 gene; humans; human; male; article; human kallikreiin related peptidase 2
Journal Title: Clinical Chemistry
Volume: 60
Issue: 3
ISSN: 0009-9147
Publisher: American Association for Clinical Chemistry  
Date Published: 2014-03-01
Start Page: 490
End Page: 499
Language: English
DOI: 10.1373/clinchem.2013.211219
PUBMED: 24270797
PROVIDER: scopus
PMCID: PMC3943614
Notes: Export Date: 1 August 2014 -- CODEN: CLCHA -- Source: Scopus
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MSK Authors
  1. Caroline Savage
    80 Savage
  2. Hans Gosta Lilja
    286 Lilja
  3. Angel M Cronin
    145 Cronin
  4. Robert J. Klein
    60 Klein