Morphologic identification of atypical chronic lymphocytic leukemia by digital microscopy Journal Article

  


Authors: Marionneaux, S.; Maslak, P.; Keohane, E. M.
Article Title: Morphologic identification of atypical chronic lymphocytic leukemia by digital microscopy
Abstract: Introduction: Atypical chronic lymphocytic leukemia (aCLL) is a morphologic variant found in approximately 25% of patients with chronic lymphocytic leukemia (CLL). Although aCLL has a more aggressive course compared to typical CLL (tCLL), it is not usually reported. This retrospective study used digital microscopy to morphologically classify CLL patients as aCLL or tCLL, and determined the prevalence of prognostic markers in each group. Methods: CellaVision AB (Lund, Sweden) was used to evaluate lymphocyte morphology on archived blood films of 97 CLL patients, and results of their prognostic marker analysis at diagnosis were obtained. The unpaired t-test, Chi-square, or Fisher's Exact test were used for statistical analysis. Results: 27% of CLL cases were morphologically classified as aCLL. The aCLL group had a higher prevalence of trisomy 12, unmutated IgVH, and CD38 expression (markers associated with poor prognosis), and a lower prevalence of 13q14 deletions compared to tCLL; this was statistically significant. Conclusion: Using digital imaging to identify aCLL is feasible, economical, and may provide clinically relevant prognostic information at diagnosis and during periodic monitoring. Further study of a larger number of patients is needed to assess the clinical utility of reporting aCLL morphology. © 2013 John Wiley & Sons Ltd.
Keywords: morphology; digital imaging; atypical chronic lymphocytic leukemia; chronic lymphocytic leukemia (cll)
Journal Title: International Journal of Laboratory Hematology
Volume: 36
Issue: 4
ISSN: 1751-5521
Publisher: Wiley Blackwell  
Date Published: 2014-08-01
Start Page: 459
End Page: 464
Language: English
DOI: 10.1111/ijlh.12167
PROVIDER: scopus
PUBMED: 24400650
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84904383885&partnerID=40&md5=a0d14c33ab4909a90395db09a0380ecb
Notes: Export Date: 1 August 2014 -- Source: Scopus
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MSK Authors
  1. Peter Maslak
    197 Maslak
  2. Steven Michael Marionneaux
    10 Marionneaux
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