SAOS-2 osteosarcoma cells bind fibroblasts via ICAM-1 and this is increased by tumour necrosis factor-α Journal Article
| Authors: | David, M. S.; Kelly, E.; Cheung, I.; Xaymardan, M.; Moore, M. A. S.; Zoellner, H. |
| Article Title: | SAOS-2 osteosarcoma cells bind fibroblasts via ICAM-1 and this is increased by tumour necrosis factor-α |
| Abstract: | We recently reported exchange of membrane and cytoplasmic markers between SAOS-2 osteosarcoma cells and human gingival fibroblasts (h-GF) without comparable exchange of nuclear markers, while similar h-GF exchange was seen for melanoma and ovarian carcinoma cells. This process of "cellular sipping" changes phenotype such that cells sharing markers of both SAOS-2 and h-GF have morphology intermediate to that of either cell population cultured alone, evidencing increased tumour cell diversity without genetic change. TNF-α increases cellular sipping between h-GF and SAOS-2, and we here study binding of SAOS-2 to TNF-α treated h-GF to determine if increased cellular sipping can be accounted for by cytokine stimulated SAOS-2 binding. More SAOS-2 bound h-GF pe-seeded wells than culture plastic alone (p<0.001), and this was increased by h-GF pre-treatment with TNF-α (p<0.001). TNF-α stimulated binding was dose dependent and maximal at 1.16nM (p<0.05) with no activity below 0.006 nM. SAOS-2 binding to h-GF was independent of serum, while the lipopolysaccharide antagonist Polymyxin B did not affect results, and TNF-α activity was lost on boiling. h- GF binding of SAOS-2 started to increase after 30min TNF-α stimulation and was maximal by 1.5hr pre-treatment (p<0.001). h-GF retained maximal binding up to 6hrs after TNF-α stimulation, but this was lost by 18hrs (p<0.001). FACS analysis demonstrated increased ICAM-1 consistent with the time course of SAOS-2 binding, while antibody against ICAM-1 inhibited SAOS-2 adhesion (p<0.04). Pre-treating SAOS-2 with TNF-α reduced h-GF binding to background levels (p<0.003), and this opposite effect to h-GF cytokine stimulation suggests that the history of cytokine exposure of malignant cells migrating across different microenvironments can influence subsequent interactions with fibroblasts. Since cytokine stimulated binding was comparable in magnitude to earlier reported TNF-α stimulated cellular sipping, we conclude that TNF-α stimulated cellular sipping likely reflects increased SAOS-2 binding as opposed to enhanced exchange mechanisms. © 2014 David et al. |
| Keywords: | controlled study; human tissue; protein expression; human cell; flow cytometry; phenotype; cell function; vascular cell adhesion molecule 1; cell population; tumor necrosis factor alpha; tumor cell; cell migration; fibroblast; cell marker; cell adhesion; intercellular adhesion molecule 1; concentration response; tumor seeding; fibroblast culture; tumor microenvironment; binding kinetics; polymyxin b; cytokine response; human; article; cell sipping; saos 2 cell line |
| Journal Title: | PLoS ONE |
| Volume: | 9 |
| Issue: | 6 |
| ISSN: | 1932-6203 |
| Publisher: | Public Library of Science |
| Date Published: | 2014-06-30 |
| Start Page: | e101202 |
| Language: | English |
| DOI: | 10.1371/journal.pone.0101202 |
| PROVIDER: | scopus |
| PMCID: | PMC4076326 |
| PUBMED: | 24979620 |
| DOI/URL: | |
| Notes: | Export Date: 1 August 2014 -- CODEN: POLNC -- Source: Scopus |
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