Tumor necrosis factor induces phosphorylation of a 28-kDa mRNA cap-binding protein in human cervical carcinoma cells Journal Article
| Authors: | Marino, M. W.; Pfeffer, L. M.; Guidon, P. T. Jr; Donner, D. B. |
| Article Title: | Tumor necrosis factor induces phosphorylation of a 28-kDa mRNA cap-binding protein in human cervical carcinoma cells |
| Abstract: | Tumor necrosis factor α (TNF-α) stimulated the phosphorylation of a 28-kDa protein (p28) in the ME-180 line of human cervical carcinoma cells. The effect of TNF-α on the phosphorylation state of p28 was rapid (4-fold increase within 15 min) and persistent, remaining above the basal level for at least 2 hr. The specific binding of 125I-labeled TNF-α to cell-surface binding sites, the stimulation of p28 phosphorylation by TNF-α, and the inhibition of cell proliferation by TNF-α occurred with nearly identical dose-response relationships. Two-dimensional SDS/PAGE resolved p28 into two isoforms having pI values of 6.2 and 6.1. A phosphorylated cap-binding protein was substantially enriched from lysates of control or TNF-α-treated ME-180 cells by affinity chromatography with 7-methylguanosine 5'-triphosphate-Sepharose. The phosphoprotein recovered from this procedure was the substrate for TNF-α-promoted phosphorylation, p28. Thus, TNF-α stimulates the phosphorylation of this mRNA cap-binding protein, which may be involved in the transduction of TNF-α-receptor binding into cellular responses. |
| Keywords: | protein phosphorylation; human cell; cell growth; tumor necrosis factor alpha; cell culture; radioisotope; uterine cervix carcinoma; cap binding protein; human; priority journal; article |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 86 |
| Issue: | 21 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1989-11-01 |
| Start Page: | 8417 |
| End Page: | 8421 |
| Language: | English |
| DOI: | 10.1073/pnas.86.21.8417 |
| PUBMED: | 2813400 |
| PROVIDER: | scopus |
| PMCID: | PMC298293 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 14 April 2020 -- Source: Scopus |
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