Derivation and characterization of mouse embryonic stem cells from permissive and nonpermissive strains Journal Article

   


Authors: Czechanski, A.; Byers, C.; Greenstein, I.; Schrode, N.; Donahue, L. R.; Hadjantonakis, A. K.; Reinholdt, L. G.
Article Title: Derivation and characterization of mouse embryonic stem cells from permissive and nonpermissive strains
Abstract: Mouse embryonic stem cells (mESCs) are key tools for genetic engineering, development of stem cell-based therapies and basic research on pluripotency and early lineage commitment. However, successful derivation of germline-competent embryonic stem cell lines has, until recently, been limited to a small number of inbred mouse strains. Recently, there have been considerable advances in the field of embryonic stem cell biology, particularly in the area of pluripotency maintenance in the epiblast from which the mESCs are derived. Here we describe a protocol for efficient derivation of germline-competent mESCs from any mouse strain, including strains previously deemed nonpermissive. We provide a protocol that is generally applicable to most inbred strains, as well as a variant for nonpermissive strains. By using this protocol, mESCs can be derived in 3 weeks and fully characterized after an additional 12 weeks, at efficiencies as high as 90% and in any strain background. © 2014 Nature America, Inc.
Keywords: protein expression; nonhuman; animal cell; mouse; cell viability; cell survival; cell structure; cell growth; embryonic stem cell; stem cell transplantation; cell differentiation; cryopreservation; genomic instability; cell count; cell density; culture technique; cell adhesion; karyotype; aneuploidy; octamer transcription factor 4; transcription factor sox2; transcription factor nanog; derivation; blastocyte; freezing; stage specific embryo antigen 1; procedures concerning cells; growth curve; zona pellucida; priority journal; article
Journal Title: Nature Protocols
Volume: 9
Issue: 3
ISSN: 1754-2189
Publisher: Nature Publishing Group  
Date Published: 2014-02-01
Start Page: 559
End Page: 574
Language: English
DOI: 10.1038/nprot.2014.030
PROVIDER: scopus
PUBMED: 24504480
PMCID: PMC4112089
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84895476817&partnerID=40&md5=56b8e9b4c9d459f0092ade7149ac8df9
Notes: Cited By (since 1996):3 -- Export Date: 1 August 2014 -- Source: Scopus
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MSK Authors
  1. Anna-Katerina Hadjantonakis
    191 Hadjantonakis
  2. Nadine Schrode
    10 Schrode
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