Immunostimulatory activities of polysaccharide extract isolated from Curcuma longa Journal Article
| Authors: | Yue, G. G. L.; Chan, B. C. L.; Hon, P. M.; Kennelly, E. J.; Yeung, S. K.; Cassileth, B. R.; Fung, K. P.; Leung, P. C.; Lau, C. B. S. |
| Article Title: | Immunostimulatory activities of polysaccharide extract isolated from Curcuma longa |
| Abstract: | Several curcuminoids and sesquiterpenoids isolated from Curcuma longa (CL) have been shown to have many pharmacological activities. In the present study, the immunomodulatory activities of the polar fractions of CL hot water extracts were investigated using human peripheral blood mononuclear cells (PBMC). Our results showed that the high polarity fraction of the hot water extract exhibited stimulatory effects on PBMC proliferation as shown in [methyl-3H]-thymidine incorporation assay. In an attempt to isolate the active components responsible for the activities, further partition with ethyl acetate, n-butanol and ethanol, progressively were performed. The cytokine productions (TGF-β, TNF-α, GM-CSF, IL-1α, IL-5, IL-6, IL-8, IL-10, IL-13, etc.) have been modulated by a polysaccharide-enriched fraction as shown in ELISA and cytokine protein array. The proportion of CD14 positive stained PBMC was increased by such fraction. The composition of monosaccharide of the active fraction has been determined by GC-MS and gel permeation chromatography. The immunostimulatory effects of C. longa polysaccharides on PBMC were shown for the first time. The findings revealed the potential use of C. longa crude extract (containing curcuminoids and polysaccharides) as an adjuvant supplement for cancer patients, whose immune activities were suppressed during chemotherapies. © 2010 Elsevier B.V. |
| Keywords: | controlled study; human cell; drug activity; nonhuman; cell proliferation; t lymphocyte; cell viability; gene expression profiling; transforming growth factor beta; granulocyte macrophage colony stimulating factor; interleukin 10; interleukin 13; interleukin 5; interleukin 8; gene expression regulation; cytokines; interleukin 6; cellular distribution; cytokine production; immunomodulation; polysaccharide; immunostimulation; t-lymphocyte subsets; antigens, cd; biological assay; mass fragmentography; adjuvants, immunologic; interleukin 1alpha; polysaccharides; molecular weight; peripheral blood mononuclear cell; curcuma longa; curcuma longa extract; curcuma; chromatography, gel; immunostimulatory; peripheral blood mononuclear cells; rhizome; gas chromatography-mass spectrometry |
| Journal Title: | International Journal of Biological Macromolecules |
| Volume: | 47 |
| Issue: | 3 |
| ISSN: | 0141-8130 |
| Publisher: | Elsevier B.V. |
| Date Published: | 2010-10-01 |
| Start Page: | 342 |
| End Page: | 347 |
| Language: | English |
| DOI: | 10.1016/j.ijbiomac.2010.05.019 |
| PUBMED: | 20609432 |
| PROVIDER: | scopus |
| PMCID: | PMC2917522 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 2" - "Export Date: 20 April 2011" - "CODEN: IJBMD" - "Source: Scopus" |
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