Discrete levels of twist activity are required to direct distinct cell functions during gastrulation and somatic myogenesis Journal Article

   

Authors:	Wong, M. C.; Dobi, K. C.; Baylies, M. K.
Article Title:	Discrete levels of twist activity are required to direct distinct cell functions during gastrulation and somatic myogenesis
Abstract:	Twist (Twi), a conserved basic helix-loop-helix transcriptional regulator, directs the epithelial-to-mesenchymal transition (EMT), and regulates changes in cell fate, cell polarity, cell division and cell migration in organisms from flies to humans. Analogous to its role in EMT, Twist has been implicated in metastasis in numerous cancer types, including breast, pancreatic and prostate. In the Drosophila embryo, Twist is essential for discrete events in gastrulation and mesodermal patterning. In this study, we derive a twi allelic series by examining the various cellular events required for gastrulation in Drosophila. By genetically manipulating the levels of Twi activity during gastrulation, we find that coordination of cell division is the most sensitive cellular event, whereas changes in cell shape are the least sensitive. Strikingly, we show that by increasing levels of Snail expression in a severe twi hypomorphic allelic background, but not a twi null background, we can reconstitute gastrulation and produce viable adult flies. Our results demonstrate that the level of Twi activity determines whether the cellular events of ventral furrow formation, EMT, cell division and mesodermal migration occur. © 2014 Wong et al.
Journal Title:	PLoS ONE
Volume:	9
Issue:	6
ISSN:	1932-6203
Publisher:	Public Library of Science
Date Published:	2014-06-10
Start Page:	e99553
Language:	English
DOI:	10.1371/journal.pone.0099553
PROVIDER:	scopus
PMCID:	PMC4051702
PUBMED:	24915423
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84902578402&partnerID=40&md5=b4453328a00f34ba8480be51b54f6dd5
Notes:	PLoS ONE -- Export Date: 8 July 2014 -- CODEN: POLNC -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/54074