Daughterless dictates Twist activity in a context-dependent manner during somatic myogenesis Journal Article
| Authors: | Wong, M. C.; Castanon, I.; Baylies, M. K. |
| Article Title: | Daughterless dictates Twist activity in a context-dependent manner during somatic myogenesis |
| Abstract: | Somatic myogenesis in Drosophila relies on the reiterative activity of the basic helix-loop-helix transcriptional regulator, Twist (Twi). How Twi directs multiple cell fate decisions over the course of mesoderm and muscle development is unclear. Previous work has shown that Twi is regulated by its dimerization partner: Twi homodimers activate genes necessary for somatic myogenesis, whereas Twi/Daughterless (Da) heterodimers lead to the repression of these genes. Here, we examine the nature of Twi/Da heterodimer repressive activity. Analysis of the Da protein structure revealed a Da repression (REP) domain, which is required for Twi/Da-mediated repression of myogenic genes, such as Dmef2, both in tissue culture and in vivo. This domain is crucial for the allocation of mesodermal cells to distinct fates, such as heart, gut and body wall muscle. By contrast, the REP domain is not required in vivo during later stages of myogenesis, even though Twi activity is required for muscles to achieve their final pattern and morphology. Taken together, we present evidence that the repressive activity of the Twi/Da dimer is dependent on the Da REP domain and that the activity of the REP domain is sensitive to tissue context and developmental timing. © 2008 Elsevier Inc. All rights reserved. |
| Keywords: | immunohistochemistry; nonhuman; protein domain; animals; basic helix-loop-helix transcription factors; drosophila; rna interference; genetic transcription; in vivo study; protein interaction; gene expression regulation, developmental; gene mapping; amino acid sequence; molecular sequence data; gene repression; base sequence; dimerization; protein structure, tertiary; dna primers; protein structure; sequence homology; transcription factor twist; mesoderm; drosophila proteins; transcriptional regulation; myocyte enhancer factor 2; gene activity; muscle; sequence analysis, dna; muscle development; tissue culture; twist transcription factor; bhlh; daughterless; twist; somites |
| Journal Title: | Developmental Biology |
| Volume: | 317 |
| Issue: | 2 |
| ISSN: | 0012-1606 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2008-05-15 |
| Start Page: | 417 |
| End Page: | 429 |
| Language: | English |
| DOI: | 10.1016/j.ydbio.2008.02.020 |
| PUBMED: | 18407256 |
| PROVIDER: | scopus |
| PMCID: | PMC2435306 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 5" - "Export Date: 17 November 2011" - "CODEN: DEBIA" - "Source: Scopus" |
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