Acoustophoresis pre-alignment of cells enables label-free enrichment of prostate cancer cells in blood Conference Paper

  


Authors: Augustsson, P.; Magnusson, C.; Lilja, H.; Laurell, T.
Title: Acoustophoresis pre-alignment of cells enables label-free enrichment of prostate cancer cells in blood
Conference Title: 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Abstract: We have developed a microfluidic chip for isolation of circulating tumor cells (CTCs) in blood based on microchannel acoustophoresis. Ultrasound radiation forces are used to separate cancer cells from blood cells in a continuous flow format. Separation was dramatically improved after incorporation of a 2-dimensional acoustic pre-alignment of the cells before entering the acoustophoresis separation channel. Temperature regulation of the microfluidic chip (±0.5?C) enabled stable and reproducible separation. The chip constitutes a further development and refinement of methods previously presented by our group at μTAS [1-3].
Keywords: ultrasound; blood; bioassay; biochips; blood cells; circulating tumor cells; separation; ultrasonics; alignment; prostate cancer cells; continuous flows; fluidic devices; microfluidic chip; separation channel; temperature regulations; ultrasound radiation force
Journal Title Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Conference Dates: 2012 Oct 28-Nov 1
Conference Location: Okinawa, Japan
ISBN: 9780979806452
Publisher: Chemical and Biological Microsystems Society  
Date Published: 2012-01-01
Start Page: 1696
End Page: 1698
Language: English
PROVIDER: scopus
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84901760395&partnerID=40&md5=01de863640fedc3050a8edf13f32eac3
Notes: Proc. Int. Conf. Miniaturized Syst. Chem. Life Sci., MicroTAS -- Conference code: 105382 -- Export Date: 8 July 2014 -- 28 October 2012 through 1 November 2012 -- Source: Scopus