Acoustic whole blood plasmapheresis chip for prostate specific antigen microarray diagnostics Journal Article
| Authors: | Lenshof, A.; Ahmad-Tajudin, A.; Järås, K.; Swärd-Nilsson, A. M.; Åberg, L.; Marko-Varga, G.; Malm, J.; Lilja, H.; Laurell, T. |
| Article Title: | Acoustic whole blood plasmapheresis chip for prostate specific antigen microarray diagnostics |
| Abstract: | The generation of high quality plasma from whole blood is of major interest for many biomedical analyses and clinical diagnostic methods. However, it has proven to be a major challenge to make use of microfluidic separation devices to process fluids with high cell content, such as whole blood. Here, we report on an acoustophoresis based separation chip that prepares diagnostic plasma from whole blood linked to a clinical application. This acoustic separator has the capacity to sequentially remove enriched blood cells in multiple steps to yield high quality plasma of low cellular content. The generated plasma fulfills the standard requirements (<6.0 × 10<sup>9</sup> erythrocytes/L) recommended by the Council of Europe. Further, we successfully linked the plasmapheresis microchip to our previously developed porous silicon sandwich antibody microarray chip for prostate specific antigen (PSA) detection. PSA was detected by good linearity (R<sup>2</sup> > 0.99) in the generated plasma via fluorescence readout without any signal amplification at clinically relevant levels (0.19-21.8 ng/mL). © 2009 American Chemical Society. |
| Keywords: | protein array analysis; nonhuman; plasmas; prostate specific antigen; fluorescence; plasmapheresis; prostate-specific antigen; prostatic neoplasms; blood; antibodies, monoclonal; antigens; microarray analysis; antigen detection; amplification; bioassay; biochips; microfluidics; cell membranes; blood cells; cell count; immunoassay; acoustics; signal processing; peeling; acoustophoresis; antibody microarrays; biomedical analysis; cellular content; clinical application; clinical diagnostics; council of europe; high quality; process fluid; separation chip; separation devices; signal amplifications; standard requirements; whole blood; porous silicon; separation; erythrocytes; microfluidic analytical techniques; silicon |
| Journal Title: | Analytical Chemistry |
| Volume: | 81 |
| Issue: | 15 |
| ISSN: | 0003-2700 |
| Publisher: | American Chemical Society |
| Date Published: | 2009-08-01 |
| Start Page: | 6030 |
| End Page: | 6037 |
| Language: | English |
| DOI: | 10.1021/ac9013572 |
| PUBMED: | 19594154 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 5" - "Export Date: 30 November 2010" - "CODEN: ANCHA" - "Source: Scopus" |
