| Abstract: |
By studying primary isogenic murine embryonic fibroblasts (MEFs), we have shown that PLK3 null MEFs contain a reduced level of phosphatase and tensin homolog (PTEN) and increased Akt1 activation coupled with decreased GSK3β activation under normoxia and hypoxia. Purified recombinant Plk3, but not a kinase-defective mutant, efficiently phosphorylates PTEN in vitro. Mass spectrometry identifies threonine 366 and serine 370 as two putative residues that are phosphorylated by Plk3. Immunoblotting using a phosphospecific antibody confirms these sites as Plk3 phosphorylation sites. Moreover, treatment of MEFs with LiCl, an inhibitor of GSK3β and CK2, only partially suppresses the phosphorylation, suggesting Plk3 as an additional kinase that phosphorylates these sites in vivo. Plk3-targeting mutants of PTEN are expressed at a reduced level in comparison with the wild-type counterpart, which is associated with an enhanced activity of PDK1, an upstream activator of Akt1. Furthermore, the reduced level of PTEN in PLK3 null MEFs is stabilized by treatment with MG132, a proteosome inhibitor. Combined, our study identifies Plk3 as a new player in the regulation of the PI3K/PDK1/Akt signaling axis by phosphorylation and stabilization of PTEN. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc. |
| Keywords: |
signal transduction; protein kinase b; controlled study; human cell; mutation; nonhuman; mass spectrometry; animal cell; mouse; animals; mice; mice, knockout; serine; embryo; in vivo study; enzyme activation; in vitro study; enzyme activity; cysteine proteinase inhibitors; phosphorylation; wild type; phosphatidylinositol 3 kinase; enzyme phosphorylation; cell culture; protein-serine-threonine kinases; glycogen synthase kinase 3beta; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase; proto-oncogene proteins c-akt; pten phosphohydrolase; wild types; murinae; immunoblotting; fibroblast; fibroblasts; embryo, mammalian; in-vivo; threonine; amino acids; adjuvants, immunologic; in-vitro; mutant; phosphoinositide dependent protein kinase 1; glycogen synthase kinase 3; chemical activation; enzyme stability; hek293 cells; leupeptins; murine embryonic fibroblasts; phosphorylation sites; phosphospecific antibodies; polo like kinase 3; lithium chloride
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