Intensity-fading MALDI-TOF-MS: Novel screening for ligand binding and drug discovery Journal Article
| Authors: | Yanes, Ó; Villanueva, J.; Querol, E.; Aviles, F. X. |
| Article Title: | Intensity-fading MALDI-TOF-MS: Novel screening for ligand binding and drug discovery |
| Abstract: | Ligand discovery technologies largely rely on a primary screening for molecules showing affinity to a target, coupled with an approach to identify these molecules. Matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) is attracting increasing attention as a suitable analytical technique for ligand identification owing to its high sensitivity and capacity for discrimination, its fast analysis and its ease of automation. There is now a range of related strategies for drug discovery, including a novel MS-based methodology to screen noncovalent interactions between macromolecular targets (proteases) and peptide or organic ligands (protease inhibitors) called intensity-fading (IF) MALDI-TOF-MS. © 2004 Elsevier Ltd. All rights reserved. |
| Keywords: | review; nonhuman; drug targeting; binding affinity; accuracy; protein analysis; protein targeting; drug screening; peptide; automation; drug research; capillary electrophoresis; matrix assisted laser desorption ionization time of flight mass spectrometry; surface plasmon resonance; proteinase inhibitor; ligand binding; protein cross linking; protein immobilization; affinity chromatography; gel permeation chromatography |
| Journal Title: | Drug Discovery Today: TARGETS |
| Volume: | 3 |
| Issue: | 2 Suppl. |
| ISSN: | 1741-8372 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2004-04-01 |
| Start Page: | S23 |
| End Page: | S30 |
| Language: | English |
| PROVIDER: | scopus |
| DOI: | 10.1016/S1741-8372(04)02417-X |
| DOI/URL: | |
| Notes: | Drug Discov. Today Targets -- Cited By (since 1996):8 -- Export Date: 16 June 2014 -- CODEN: DDTTA -- Source: Scopus |
