i-SNAREs: Inhibitory SNAREs that fine-tune the specificity of membrane fusion Journal Article
| Authors: | Varlamov, O.; Volchuk, A.; Rahimian, V.; Doege, C. A.; Paumet, F.; Eng, W. S.; Arango, N.; Parlati, F.; Ravazzola, M.; Orci, L.; Sollner, T. H.; Rothman, J. E. |
| Article Title: | i-SNAREs: Inhibitory SNAREs that fine-tune the specificity of membrane fusion |
| Abstract: | A new functional class of SNAREs, designated inhibitory SNAREs (i-SNAREs), is described here. An i-SNARE inhibits fusion by substituting for or binding to a subunit of a fusogenic SNAREpin to form a nonfusogenic complex. Golgi-localized SNAREs were tested for i-SNARE activity by adding them as a fifth SNARE together with four other SNAREs that mediate Golgi fusion reactions. A striking pattern emerges in which certain subunits of the cis-Golgi SNAREpin function as i-SNAREs that inhibit fusion mediated by the trans-Golgi SNAREpin, and vice versa. Although the opposing distributions of the cis- and trans-Golgi SNAREs themselves could provide for a countercurrent fusion pattern in the Golgi stack, the gradients involved would be strongly sharpened by the complementary countercurrent distributions of the i-SNAREs. |
| Keywords: | signal transduction; unclassified drug; protein function; protein localization; complex formation; protein binding; membrane proteins; protein transport; binding sites; regulation; protein subunit; protein subunits; cis isomer; trans isomer; cell secretion; golgi complex; vesicular transport proteins; intracellular membranes; membrane fusion; snare protein; vesicles; golgi apparatus; snare proteins; golgi; gradient; trans-golgi network; priority journal; article; homotypic; inhibitory snare protein |
| Journal Title: | Journal of Cell Biology |
| Volume: | 164 |
| Issue: | 1 |
| ISSN: | 0021-9525 |
| Publisher: | Rockefeller University Press |
| Date Published: | 2004-01-05 |
| Start Page: | 79 |
| End Page: | 88 |
| Language: | English |
| DOI: | 10.1083/jcb.200307066 |
| PROVIDER: | scopus |
| PMCID: | PMC2171956 |
| PUBMED: | 14699088 |
| DOI/URL: | |
| Notes: | J. Cell Biol. -- Cited By (since 1996):40 -- Export Date: 16 June 2014 -- CODEN: JCLBA -- Source: Scopus |
