Countercurrent distribution of two distinct SNARE complexes mediating transport within the Golgi stack Journal Article

   


Authors: Volchuk, A.; Ravazzola, M.; Perrelet, A.; Eng, W. S.; Di Liberto, M.; Varlamov, O.; Fukasawa, M.; Engel, T.; Söllner, T. H.; Rothman, J. E.; Orci, L.
Article Title: Countercurrent distribution of two distinct SNARE complexes mediating transport within the Golgi stack
Abstract: Genetic and biochemical evidence has established that a SNARE complex consisting of syntaxin 5 (Sed5)-mYkt6 (Ykt6)-GOS28 (Gos1)-GS15 (Sft1) is required for transport of proteins across the Golgi stack in animals (yeast). We have utilized quantitative immunogold labeling to establish the cis-trans distribution of the v-SNARE GS15 and the t-SNARE subunits GOS28 and syntaxin 5. Whereas the distribution of the t-SNARE is nearly even across the Golgi stack from the cis to the trans side, the v-SNARE GS15 is present in a gradient of increasing concentration toward the trans face of the stack. This contrasts with a second distinct SNARE complex, also required for intra-Golgi transport, consisting of syntaxin 5 (Sed5)-membrin (Bos1)-ERS24 (Sec22)-rBet1 (Bet1), whose v- (rBet1) and t-SNARE subunits (membrin and ERS24), progressively decrease in concentration toward the trans face. Transport within the stack therefore appears to utilize countercurrent gradients of two Golgi SNAREpins and may involve a mechanism akin to homotypic fusion.
Keywords: immunohistochemistry; controlled study; unclassified drug; nonhuman; protein function; protein localization; animal cell; animals; microscopy, electron; complex formation; heredity; models, biological; cell line; membrane proteins; dose-response relationship, drug; hela cells; animalia; kidney; quantitative analysis; carrier proteins; rat; cell membrane; protein transport; cellular distribution; microscopy, fluorescence; rats; yeast; protein subunit; biological transport; golgi complex; vesicular transport proteins; snare protein; syntaxin; cell-free system; membrin; syntaxin 5; qa-snare proteins; qb-snare proteins; golgi apparatus; r-snare proteins; snare proteins; immunogold labeling; precipitin tests; humans; priority journal; article; protein ers24; protein gos28; protein rbet1; qc-snare proteins
Journal Title: Molecular Biology of the Cell
Volume: 15
Issue: 4
ISSN: 1059-1524
Publisher: The American Society for Cell Biology  
Date Published: 2004-04-01
Start Page: 1506
End Page: 1518
Language: English
DOI: 10.1091/mbc.E03-08-0625
PROVIDER: scopus
PMCID: PMC379251
PUBMED: 14742712
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-12144290286&partnerID=40&md5=dd3c3218c514026039f10ffd9debed9c
Notes: Mol. Biol. Cell -- Cited By (since 1996):44 -- Export Date: 16 June 2014 -- CODEN: MBCEE -- Source: Scopus
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MSK Authors
  1. Oleg Varlamov
    8 Varlamov
  2. Thomas H Sollner
    65 Sollner
  3. James E Rothman
    120 Rothman
  4. Masayoshi Fukasawa
    2 Fukasawa
  5. Allen Volchuk
    10 Volchuk
  6. Thomas A Engel
    4 Engel
  7. Maurizio Diliberto
    6 Diliberto
  8. William S Eng
    5 Eng
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