Wnt1 overexpression leads to enforced cardiomyogenesis and inhibition of hematopoiesis in murine embryonic stem cells Journal Article
| Authors: | Weisel, K. C.; Kopp, H. G.; Moore, M. A. S.; Studer, L.; Barberi, T. |
| Article Title: | Wnt1 overexpression leads to enforced cardiomyogenesis and inhibition of hematopoiesis in murine embryonic stem cells |
| Abstract: | Recent findings emphasized a critical role for the Wnt signaling pathway during the early steps of embryogenesis, including the development of the hematopoietic system and cardiac development. To date, the role of Wnt in promoting or inhibiting development of both tissues was discussed controversially, dependent on species and time point of expression. Differentiation of embryonic stem cells (ESC) recapitulates early stages of mammalian development. In the present study, we generated murine ESC lines overexpressing Wnt1 (Wnt1 ES). When induced to differentiate toward the cardiomyocytic lineage, Wnt1 ES showed a significant increased ability to generate cardiomyocytes when compared with a control ESC (control ES) line. In addition, Wnt1 ES cells were unable to form hematopoietic cells, whereas development of endothelial cells, a cell type closely associated with blood during embryogenesis, was comparable to control ES. Finally, cardiac differentiation was markedly decreased by the addition of the Wnt antagonist Dkk-1 to the culture medium. These findings suggest that Wnt1 may regulate differentiation of immature mesodermal cells in a tissue-specific manner. © 2010, Mary Ann Liebert, Inc. |
| Keywords: | signal transduction; controlled study; nonhuman; protein function; animal cell; mouse; mammalia; animals; mice; gene overexpression; embryo; embryonic stem cell; culture medium; cell line; embryo development; cell differentiation; cell lineage; cell type; endothelium cell; hematopoietic cell; murinae; embryonic stem cells; inhibition kinetics; hematopoiesis; wnt protein; mesoderm; developmental stage; wnt1 protein; heart development; frizzled protein; tissue specificity; muscle development; dickkopf 1 protein; low density lipoprotein receptor related protein 5; low density lipoprotein receptor related protein 6; myocytes, cardiac |
| Journal Title: | Stem Cells and Development |
| Volume: | 19 |
| Issue: | 5 |
| ISSN: | 1547-3287 |
| Publisher: | Mary Ann Liebert, Inc |
| Date Published: | 2010-05-01 |
| Start Page: | 745 |
| End Page: | 751 |
| Language: | English |
| DOI: | 10.1089/scd.2008.0356 |
| PUBMED: | 19422288 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Export Date: 20 April 2011" - "CODEN: SCDTA" - "Source: Scopus" |
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