Laser microdissection and molecular typing of dysplastic cells from Pap smears: A new approach to early detection of cervical cancer Journal Article
| Authors: | Eder, C.; Chaganti, R. S. K.; Murty, V. V. V. S.; Greskötter, K. R.; Giesing, M. |
| Article Title: | Laser microdissection and molecular typing of dysplastic cells from Pap smears: A new approach to early detection of cervical cancer |
| Abstract: | The Papanicolaou smear (Pap) is a worldwide screening tool for early detection of cervical cancer and its precursor lesions. The transition from dysplasia to cancer is a highly complex genetic process and cannot be predicted based solely on cell morphology. Molecular characterization of precursor lesions could yield a better definition of lesions at high risk for progression. We developed an analytical concept comprising not only morphological characterization but also molecular analysis with multiple parameters of dysplastic cells from cervical smears. We isolated dysplastic cells from 52 fixed Pap-stained smears of various grades by laser microdissection and analyzed them for genetic lesions typical for cervical carcinoma. The loss of heterozygosity (LOH) as published for cervical carcinoma tissue was detected. Markers for early stages showed a LOH in 43% and 22%, and those for late stages in 26% of the cases. Combining morphological characterization with molecular ana lysis by multiple molecular markers could open up new opportunities for early detection of cervical carcinoma. |
| Keywords: | human cell; cancer diagnosis; cancer screening; morphology; early diagnosis; lasers; molecular typing; cell isolation; heterozygosity loss; high risk population; loss of heterozygosity; uterine cervical neoplasms; genetic markers; uterine cervix carcinoma; dysplasia; vaginal smears; microdissection; laser microdissection; papanicolaou test; cell ultrastructure; cervical carcinoma; humans; human; female; article; dysplastic cells; loh analysis; pap smear |
| Journal Title: | Pathologe |
| Volume: | 25 |
| Issue: | 3 |
| ISSN: | 0172-8113 |
| Publisher: | Springer Verlag |
| Date Published: | 2004-05-01 |
| Start Page: | 209 |
| End Page: | 215 |
| Language: | German |
| DOI: | 10.1007/s00292-003-0663-4 |
| PROVIDER: | scopus |
| PUBMED: | 15138702 |
| DOI/URL: | |
| Notes: | Pathologe -- Cited By (since 1996):2 -- Export Date: 16 June 2014 -- CODEN: PATHD -- Source: Scopus |
