Essential role of Plzf in maintenance of spermatogonial stem cells Journal Article

  


Authors: Costoya, J. A.; Hobbs, R. M.; Barna, M.; Cattoretti, G.; Manova, K.; Sukhwani, M.; Orwig, K. E.; Wolgemuth, D. J.; Pandolfi, P. P.
Article Title: Essential role of Plzf in maintenance of spermatogonial stem cells
Abstract: Little is known of the molecular mechanisms whereby spermatogonia, mitotic germ cells of the testis, self-renew and differentiate into sperm. Here we show that Zfp145, encoding the transcriptional repressor Plzf, has a crucial role in spermatogenesis. Zfp145 expression was restricted to gonocytes and undifferentiated spermatogonia and was absent in tubules of W/Wv mutants that lack these cells. Mice lacking Zfp145 underwent a progressive loss of spermatogonia with age, associated with increases in apoptosis and subsequent loss of tubule structure but without overt differentiation defects or loss of the supporting Sertoli cells. Spermatogonial transplantation experiments revealed a depletion of spermatogonial stem cells in the adult. Microarray analysis of isolated spermatogonia from Zfp145-null mice before testis degeneration showed alterations in the expression profile of genes associated with spermatogenesis. These results identify Plzf as a spermatogonia-specific transcription factor in the testis that is required to regulate self-renewal and maintenance of the stem cell pool.
Keywords: controlled study; unclassified drug; dna-binding proteins; nonhuman; animal cell; mouse; animals; mice; mice, knockout; gene; apoptosis; gene expression; gene product; gene function; cell differentiation; animalia; transcription factors; stem cell; molecular mechanics; epigenesis, genetic; cell isolation; stem cells; dna microarray; genetic code; testis; spermatogenesis; protein plzf; sertoli cell; spermatogonia; spermatogonium; male; priority journal; article; gene zfp145
Journal Title: Nature Genetics
Volume: 36
Issue: 6
ISSN: 1061-4036
Publisher: Nature Publishing Group  
Date Published: 2004-06-01
Start Page: 653
End Page: 659
Language: English
DOI: 10.1038/ng1367
PROVIDER: scopus
PUBMED: 15156143
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-2642550555&partnerID=40&md5=4897e35e160eff8f4361ed463b852590
Notes: Nat. Genet. -- Cited By (since 1996):346 -- Export Date: 16 June 2014 -- CODEN: NGENE -- Source: Scopus
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MSK Authors
  1. Robin Mark Hobbs
    21 Hobbs
  2. Jose A Costoya
    11 Costoya
  3. Pier Paolo Pandolfi Derinaldis
    353 Pandolfi Derinaldis
  4. Katia O Manova-Todorova
    161 Manova-Todorova
  5. Maria Barna
    7 Barna
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