Repression of kit expression by Plzf in germ cells Journal Article
| Authors: | Filipponi, D.; Hobbs, R. M.; Ottolenghi, S.; Rossi, P.; Jannini, E. A.; Pandolfi, P. P.; Dolci, S. |
| Article Title: | Repression of kit expression by Plzf in germ cells |
| Abstract: | Male mice lacking expression of Plzf, a DNA sequence-specific transcriptional repressor, show progressive germ cell depletion due to exhaustion of the spermatogonial stem cell population. This is likely due to the deregulated expression of genes controlling the switch between spermatogonial self-renewal and differentiation. Here we show that Plzf directly represses the transcription of kit, a hallmark of spermatogonial differentiation. Plzf represses both endogenous kit expression and expression of a reporter gene under the control of the kit promoter region. A discrete sequence of the kit promoter, required for Plzf-mediated kit transcriptional repression, is bound by Plzf both in vivo and in vitro. A 3-bp mutation in this Plzf binding site abolishes the responsiveness of the kit promoter to Plzf repression. A significant increase in kit expression is also found in the undifferentiated spermatogonia isolated from Plzf-/- mice. Thus, we suggest that one mechanism by which Plzf maintains the pool of spermatogonial stem cells is through a direct repression of kit expression. Copyright © 2007, American Society for Microbiology. All Rights Reserved. |
| Keywords: | controlled study; protein expression; unclassified drug; human cell; promoter region; nonhuman; protein function; animal cell; mouse; animals; mice; gene; mus; proto-oncogene proteins c-kit; germ cell; cell line; protein binding; transcription factor; gene function; cell differentiation; mice, transgenic; gene expression regulation; germ cells; transcription regulation; cell strain hek293; kruppel-like transcription factors; reporter gene; binding site; genes, reporter; octamer transcription factor-3; kit gene; base mispairing; spermatogenesis; spermatogonia; consensus sequence; spermatogonium; transcription factor plzf; promoter regions (genetics) |
| Journal Title: | Molecular and Cellular Biology |
| Volume: | 27 |
| Issue: | 19 |
| ISSN: | 0270-7306 |
| Publisher: | American Society for Microbiology |
| Date Published: | 2007-10-01 |
| Start Page: | 6770 |
| End Page: | 6781 |
| Language: | English |
| DOI: | 10.1128/mcb.00479-07 |
| PUBMED: | 17664282 |
| PROVIDER: | scopus |
| PMCID: | PMC2099235 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 32" - "Export Date: 17 November 2011" - "CODEN: MCEBD" - "Source: Scopus" |
