Cytosol-derived proteins are sufficient for Arp2/3 recruitment and ARF/coatomer-dependent actin polymerization on Golgi membranes Journal Article

  

Authors:	Chen, J. L.; Lacomis, L.; Erdjument-Bromage, H.; Tempst, P.; Stamnes, M.
Article Title:	Cytosol-derived proteins are sufficient for Arp2/3 recruitment and ARF/coatomer-dependent actin polymerization on Golgi membranes
Abstract:	The actin cytoskeleton has been implicated in protein trafficking at the Golgi apparatus and in Golgi orientation and morphology. Actin dynamics at the Golgi are regulated in part by recruiting Cdc42 or Rac to the membrane through a binding interaction with the coatomer-coated (COPI)-vesicle coat protein, coatomer. This leads to actin polymerization through the effector, N-WASP and the Arp2/3 complex. Here, we have used reconstitution of vesicle budding to test whether Arp2/3 is recruited to membranes during the formation of COPI vesicles. Our results revealed that ARF1 activation leads to greatly increased Arp3 levels on the membranes. Coatomer-bound Cdc42 and pre-existing F-actin are important for Arp2/3 binding. ARF1-dependent Arp2/3 recruitment and actin polymerization can be reconstituted on liposomal membranes, indicating that no membrane proteins are necessary. These results show that activated ARF1 can stimulate Arp2/3 recruitment to Golgi membranes through coatomer, Cdc42 or Rac, and N-WASP. © 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
Keywords:	controlled study; nonhuman; animals; nerve tissue proteins; protein binding; liver; bacterial proteins; regulatory mechanism; brain; recombinant proteins; rat; cattle; rats; budding; arf protein; protein structure; actins; cytoskeleton; cytosol; liposomes; protein cdc42; protein polymerization; wiskott-aldrich syndrome protein, neuronal; cytoskeletal proteins; golgi complex; cdc42; rabbits; membrane; intracellular membranes; coatomer protein; cop-coated vesicles; adp-ribosylation factor; bacterial toxins; cdc42 gtp-binding protein; cytotoxins; golgi apparatus; arp2/3; actin related protein 2; actin related protein 3; adp-ribosylation factor 1; coatomer; priority journal; article; actin-related protein 2; actin-related protein 3
Journal Title:	FEBS Letters
Volume:	566
Issue:	1-3
ISSN:	0014-5793
Publisher:	Wiley Blackwell
Date Published:	2004-05-01
Start Page:	281
End Page:	286
Language:	English
DOI:	10.1016/j.febslet.2004.04.061
PROVIDER:	scopus
PUBMED:	15147909
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-2442494215&partnerID=40&md5=24611a440b2aef40d445683b4c4ba77a
Notes:	FEBS Lett. -- Cited By (since 1996):33 -- Export Date: 16 June 2014 -- CODEN: FEBLA -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/53177