POT1-interaction protein PIP1: A telomere length regulator that recruits POT1 to the TIN2/TRF1 complex Journal Article


Authors: Ye, Z. S. J. ; Hockemeyer, D.; Krutchinsky, A. N.; Loayza, D.; Hooper, S. M.; Chait, B. T.; De Lange, T.
Article Title: POT1-interaction protein PIP1: A telomere length regulator that recruits POT1 to the TIN2/TRF1 complex
Abstract: Human telomere length is controlled by a negative feedback loop based on the binding of TRF1 to double-stranded telomeric DNA. The TRF1 complex recruits POT1, a single-stranded telomeric DNA-binding protein necessary for cis-inhibition of telomerase. By mass spectrometry, we have identified a new telomeric protein, which we have named POT1-interacting protein 1 (PIP1). PIP1 bound both POT1 and the TRF1-interacting factor TIN2 and could tether POT1 to the TRF1 complex. Reduction of PIP1 or POT1 levels with shRNAs led to telomere elongation, indicating that PIP1 contributes to telomere length control through recruitment of POT1.
Keywords: controlled study; protein expression; carrier protein; unclassified drug; dna binding protein; human cell; comparative study; protein function; mass spectrometry; telomere; metabolism; complex formation; embryo; protein protein interaction; protein; protein binding; rna interference; hela cell; hela cells; telomerase; physiology; rna; molecular cloning; cloning, molecular; fluorescent antibody technique; signal peptide; intracellular signaling peptides and proteins; nucleotide sequence; carrier proteins; negative feedback; two hybrid system; telomeric repeat binding factor 1; two-hybrid system techniques; reduction; telomere-binding proteins; telomere binding protein; pot1; humans; human; priority journal; article; protein pot1; telomeric repeat binding protein 1; pip1; tin2; trf1; pot1 interacting protein 1; protein tin2 trf1; pak1ip1 protein, human; pot1 protein, human; tinf2 protein, human
Journal Title: Genes and Development
Volume: 18
Issue: 14
ISSN: 0890-9369
Publisher: Cold Spring Harbor Laboratory Press  
Date Published: 2004-07-01
Start Page: 1649
End Page: 1654
Language: English
DOI: 10.1101/gad.1215404
PROVIDER: scopus
PMCID: PMC478187
PUBMED: 15231715
DOI/URL:
Notes: Genes Dev. -- Cited By (since 1996):204 -- Export Date: 16 June 2014 -- CODEN: GEDEE -- Molecular Sequence Numbers: GENBANK: BC016904; -- Source: Scopus
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  1. Zheng-Sheng J Ye
    4 Ye