A basis for SUMO protease specificity provided by analysis of human Senp2 and a Senp2-SUMO complex Journal Article
| Authors: | Reverter, D.; Lima, C. D. |
| Article Title: | A basis for SUMO protease specificity provided by analysis of human Senp2 and a Senp2-SUMO complex |
| Abstract: | Modification of cellular proteins by the ubiquitin-like protein SUMO is essential for nuclear metabolism and cell cycle progression in yeast. X-ray structures of the human Senp2 catalytic protease domain and of a covalent thiohemiacetal transition-state complex obtained between the Senp2 catalytic domain and SUMO-1 revealed details of the respective protease and substrate surfaces utilized in interactions between these two proteins. Comparative biochemical and structural analysis between Senp2 and the yeast SUMO protease Ulp1 revealed differential abilities to process SUMO-1, SUMO-2, and SUMO-3 in maturation and deconjugation reactions. Further biochemical characterization of the three SUMO isoforms into which an additional Gly-Gly di-peptide was inserted, or whereby the respective SUMO tails from the three isoforms were swapped, suggests a strict dependence for SUMO isopeptidase activity on residues C-terminal to the conserved Gly-Gly motif and preferred cleavage site for SUMO proteases. |
| Keywords: | unclassified drug; protein motif; protein analysis; cell cycle; carboxy terminal sequence; cell protein; protein binding; cloning, molecular; amino acid sequence; molecular sequence data; sequence homology, amino acid; models, molecular; crystallography, x-ray; yeast; protein structure; enzyme specificity; sumo protein; catalytic domain; isoprotein; glycine; small ubiquitin-related modifier proteins; enzyme active site; x ray analysis; yeasts; maturation; sumo 1 protein; cysteine endopeptidases; humans; priority journal; article; small ubiquitin related modifier 2; small ubiquitin related modifier 3 |
| Journal Title: | Structure |
| Volume: | 12 |
| Issue: | 8 |
| ISSN: | 0969-2126 |
| Publisher: | Cell Press |
| Date Published: | 2004-08-01 |
| Start Page: | 1519 |
| End Page: | 1531 |
| Language: | English |
| DOI: | 10.1016/j.str.2004.05.023 |
| PROVIDER: | scopus |
| PUBMED: | 15296745 |
| DOI/URL: | |
| Notes: | Structure -- Cited By (since 1996):95 -- Export Date: 16 June 2014 -- CODEN: STRUE -- Source: Scopus |
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